The complete genome and phenome of a community-acquired Acinetobacter baumannii

Daniel Farrugia, Liam Elbourne, Karl Hassan, Bart Eijkelkamp, Sasha Tetu, Melissa Brown, Bhumika Shah, Anton Peleg, Bridget Mabbutt, Ian Paulsen

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    Abstract

    Many sequenced strains of Acinetobacter baumannii are established nosocomial pathogens capable of resistance to multiple antimicrobials. Community-acquired A. baumannii in contrast, comprise a minor proportion of all A. baumannii infections and are highly susceptible to antimicrobial treatment. However, these infections also present acute clinical manifestations associated with high reported rates of mortality. We report the complete 3.70 Mbp genome of A. baumannii D1279779, previously isolated from the bacteraemic infection of an Indigenous Australian; this strain represents the first community-acquired A. baumannii to be sequenced. Comparative analysis of currently published A. baumannii genomes identified twenty-four accessory gene clusters present in D1279779. These accessory elements were predicted to encode a range of functions including polysaccharide biosynthesis, type I DNA restriction-modification, and the metabolism of novel carbonaceous and nitrogenous compounds. Conversely, twenty genomic regions present in previously sequenced A. baumannii strains were absent in D1279779, including gene clusters involved in the catabolism of 4-hydroxybenzoate and glucarate, and the A. baumannii antibiotic resistance island, known to bestow resistance to multiple antimicrobials in nosocomial strains. Phenomic analysis utilising the Biolog Phenotype Microarray system indicated that A. baumannii D1279779 can utilise a broader range of carbon and nitrogen sources than international clone I and clone II nosocomial isolates. However, D1279779 was more sensitive to antimicrobial compounds, particularly beta-lactams, tetracyclines and sulphonamides. The combined genomic and phenomic analyses have provided insight into the features distinguishing A. baumannii isolated from community-acquired and nosocomial infections.

    Original languageEnglish
    Article numbere58628
    Pages (from-to)e58628
    Number of pages13
    JournalPLoS One
    Volume8
    Issue number3
    DOIs
    Publication statusPublished - 19 Mar 2013

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    Farrugia, D., Elbourne, L., Hassan, K., Eijkelkamp, B., Tetu, S., Brown, M., Shah, B., Peleg, A., Mabbutt, B., & Paulsen, I. (2013). The complete genome and phenome of a community-acquired Acinetobacter baumannii. PLoS One, 8(3), e58628. [e58628]. https://doi.org/10.1371/journal.pone.0058628