The complete mitochondrial genome analysis of the tiger (Panthera tigris)

Thitika Kitpipit, Shanan Tobe, Adrian Linacre

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    11 Citations (Scopus)

    Abstract

    The complete mitochondrial genomes of five tiger samples from three subspecies (P. t. sumatrae, P. t. altica, and P. t. tigris) were successfully obtained by using 26 specifically designed Panthera-specific primer sets. The genome organization and gene arrangement of the five tiger samples were similar to each other; however polymorphic tandem repeat sequences were observed in the control region (CR). This led to a difference in the genome lengths obtained from these five samples with an average size of 16,994 bp for the five tiger mitochondrial genomes. The nucleotide base composition was on average as follows: A, 31.8%; T, 27.0%; C, 26.6%; G, 14.6% and exhibited compositional asymmetry. Most of tiger mitochondrial genome characteristics are similar to those of other common vertebrate species; however, some distinctive features were observed in the CR. First, the repetitive sequence 2 (RS 2) contained two repeat units of 80 bp and the first 15 bp of what would be the third repeat motif. The repetitive sequence 3 (RS 3) contained 47-50 repeat motifs of a shorter 8 bp (ACGTAYAC)n. Second, length heteroplasmy polycystosine (poly-C) stretches was observed at the end of the HV I locus in all tiger samples.

    Original languageEnglish
    Pages (from-to)5745-5754
    Number of pages10
    JournalMolecular Biology Reports
    Volume39
    Issue number5
    DOIs
    Publication statusPublished - May 2012

    Keywords

    • Complete mitochondrial genome
    • Genome characterization
    • Tiger (Panthera tigris)

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