TY - JOUR
T1 - The development of 20 microsatellite loci for the Australian marine mollusk, Donax deltoides, through next generation DNA sequencing
AU - Miller, Adam
AU - Versace, Vincent
AU - Matthews, Ty
AU - Bowie, Kate
PY - 2012
Y1 - 2012
N2 - Next Generation DNA sequencing was used to develop a suite of microsatellite markers for the marine mollusk, Donax deltoides. A total of 20 polymorphic loci were identified and 12 characterized using 30 individuals from a single population (Venus Bay) in south eastern Australia. We observed moderate to high genetic variation across most loci (mean number of alleles per locus = 7.3; mean heterozygosity = 0.633) with only a single locus (Ddel32) displaying significant deviation from Hardy-Weinberg equilibrium. Marker independence was confirmed with tests for linkage disequilibrium, however two loci were found to be influenced by null alleles. The 10 viable markers characterized in the present study provide a valuable resource for future population genetic assessments and fisheries management of D. deltoides in Australia.
AB - Next Generation DNA sequencing was used to develop a suite of microsatellite markers for the marine mollusk, Donax deltoides. A total of 20 polymorphic loci were identified and 12 characterized using 30 individuals from a single population (Venus Bay) in south eastern Australia. We observed moderate to high genetic variation across most loci (mean number of alleles per locus = 7.3; mean heterozygosity = 0.633) with only a single locus (Ddel32) displaying significant deviation from Hardy-Weinberg equilibrium. Marker independence was confirmed with tests for linkage disequilibrium, however two loci were found to be influenced by null alleles. The 10 viable markers characterized in the present study provide a valuable resource for future population genetic assessments and fisheries management of D. deltoides in Australia.
KW - Donax deltoides
KW - Marine mollusk
KW - Microsatellite development
KW - Next generation DNA sequencing
UR - http://www.scopus.com/inward/record.url?scp=84871261292&partnerID=8YFLogxK
U2 - 10.1007/s12686-011-9519-z
DO - 10.1007/s12686-011-9519-z
M3 - Article
VL - 4
SP - 257
EP - 260
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
SN - 1877-7252
IS - 2
ER -