The diversity of the Endophytic Actinobacteria in Australian rice plants affected by plant genotypes and soil types

Fitri Widiantini, Vadakattu V.S.R. Gupta, Christopher M.M. Franco

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)


Following the finding that Microbispora are the dominant genus of Actinobacteria in rice (Oryza sativa L.) plants grown in a Vertisol soil from Yanco, NSW, Australia, it was decided to test the hypothesis whether this observation was t rice cultivar- or soil-dependent. Four rice cultivars grown in four agricultural soils were subjected to comparative assessment of their root and shoot endophytic actinobacterial population diversity, employing a classical culture-dependent approach as well as 16S rRNA gene PCR T-RFLP as the culture-independent method. The four rice cultivars grown in four types of inundated soil were harvested after 10 weeks, and the roots and shoots surface-sterilised followed by isolation of actinobacteria or extraction of community DNA for 16S rRNA gene PCR T-RFLP. The TRFs and the association of plant genotypes and soil types on the endophytic actinobacterial diversity were identified and analysed using MiCA3 and multidimensional scaling (MDS), respectively. The majority of endophytic actinobacteria isolated from rice plants belonged to the genus Microbispora. Complementary results were detected by T-RFLP as the TRFs combination of HhaI-RsaI digestion related to Microbispora was found associated with all plant samples and in some cases was present at a relatively high percentage. Furthermore, the MDS bi-plot ordination demonstrated that the endophytic actinobacterial population was significantly affected both by plant genotype and soil type with the effect of soil type stronger (CV=24%) than the effect of plant genotype (CV=18%).

Original languageEnglish
Title of host publicationOryza sativa
Subtitle of host publicationProduction, Cultivation and Uses
EditorsHerman Kearns
Place of PublicationNew York
PublisherNova Science Publishers, Inc.
Number of pages44
ISBN (Electronic)9781536192568
Publication statusPublished - 29 Jan 2021

Bibliographical note

Publisher Copyright:
© 2021 by Nova Science Publishers, Inc.


  • Actinobacterial endophytes
  • Rice
  • Rice cultivars
  • Soil type
  • T-RFLP
  • TRFs


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