The DNase I generated disomal series is coherent to 16N Implications for coiling models of chromatin structure

S. J. Davis; L. A. Burgoyne

doi:10.1016/0014-5793(87)80556-2

The DNase I generated disomal series is coherent to 16N Implications for coiling models of chromatin structure

S. J. Davis, L. A. Burgoyne

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end-labelling and field-inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible with the most structurally acceptable coiling models. We propose that this is evidence for the general unsuitability of coiling models and is support for the existence of simple "back-to-back" double-stranded structures within chromatin.

Original language	English
Pages (from-to)	88-90
Number of pages	3
Journal	FEBS Letters
Volume	226
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80556-2
Publication status	Published - 21 Dec 1987

Keywords

Chromatin
Disomal series
DNase I
Field-inversion gel electrophoresis
Structural model

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AB - The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end-labelling and field-inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible with the most structurally acceptable coiling models. We propose that this is evidence for the general unsuitability of coiling models and is support for the existence of simple "back-to-back" double-stranded structures within chromatin.

KW - Chromatin

KW - Disomal series

KW - DNase I

KW - Field-inversion gel electrophoresis

KW - Structural model

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The DNase I generated disomal series is coherent to 16N Implications for coiling models of chromatin structure

Abstract

Keywords

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