The effect of bone marrow stromal cells on the cloning of human leukemia/lymphoma cell lines

Ram Seshadri; Chris Matthews; Cristos Gardiakos; Alexander A. Morley

doi:10.1002/stem.5530020501

The effect of bone marrow stromal cells on the cloning of human leukemia/lymphoma cell lines

Ram Seshadri, Chris Matthews, Cristos Gardiakos, Alexander A. Morley

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16 Citations (Scopus)

Abstract

The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. In general, the cloning efficiency in microwells was greater than in soft agar. The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells.

Original language	English
Pages (from-to)	277-284
Number of pages	8
Journal	The International Journal of Cell Cloning
Volume	2
Issue number	5
DOIs	https://doi.org/10.1002/stem.5530020501
Publication status	Published - 1984

Keywords

Bone marrow stromal cells
Cloning
Limiting dilution
Lymphoma cell lines

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title = "The effect of bone marrow stromal cells on the cloning of human leukemia/lymphoma cell lines",

abstract = "The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. In general, the cloning efficiency in microwells was greater than in soft agar. The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells.",

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AU - Seshadri, Ram

AU - Matthews, Chris

AU - Gardiakos, Cristos

AU - Morley, Alexander A.

PY - 1984

Y1 - 1984

N2 - The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. In general, the cloning efficiency in microwells was greater than in soft agar. The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells.

AB - The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. In general, the cloning efficiency in microwells was greater than in soft agar. The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells.

KW - Bone marrow stromal cells

KW - Cloning

KW - Limiting dilution

KW - Lymphoma cell lines

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JO - The International Journal of Cell Cloning

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The effect of bone marrow stromal cells on the cloning of human leukemia/lymphoma cell lines

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Keywords

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