The effect of bone marrow stromal cells on the cloning of human leukemia/lymphoma cell lines

Ram Seshadri, Chris Matthews, Cristos Gardiakos, Alexander A. Morley

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. In general, the cloning efficiency in microwells was greater than in soft agar. The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells.

    Original languageEnglish
    Pages (from-to)277-284
    Number of pages8
    JournalThe International Journal of Cell Cloning
    Volume2
    Issue number5
    DOIs
    Publication statusPublished - 1984

    Keywords

    • Bone marrow stromal cells
    • Cloning
    • Limiting dilution
    • Lymphoma cell lines

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