The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children.

Isabel Ordiz, Thaddaeus May, Kathie Mihindukulasuriya, John Martin, Jan Crowley, Phillip Tarr, Kelsey Ryan, Elissa Mortimer, Geetha Gopalsamy, Ken Maleta, Makedonka Mitreva, Graeme Young, Mark Manary

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    Background

    Resistant starch (RS) decreases intestinal inflammation in some settings. We tested the hypothesis that gut inflammation will be reduced with dietary supplementation with RS in rural Malawian children. Eighteen stunted 3–5-year-old children were supplemented with 8.5 g/day of RS type 2 for 4 weeks. The fecal samples were analyzed for the microbiota, the microbiome, short chain fatty acids, metabolome, and proteins indicative of inflammation before and after the intervention. Subjects served as their own controls.

    Results

    The consumption of RS changed the composition of the microbiota; at the phylum level Actinobacteria increased, while Firmicutes decreased. Among the most prevalent genera, Lactobacillus was increased and RoseburiaBlautia, and Lachnospiracea incertae sedis were decreased. The Shannon H index at the genus level decreased from 2.02 on the habitual diet and 1.76 after the introduction of RS (P < 0.01). Fecal acetate concentration decreased, and fecal propionate concentration increased after RS administration (−5.2 and 2.0 μmol/g, respectively). Fecal calprotectin increased from 29 ± 69 to 89 ± 49 μg/g (P = 0.003) after RS was given. The lipopolysaccharide biosynthesis pathway was upregulated.

    Conclusions

    Our findings do not support the hypothesis that RS reduces gut inflammation in rural Malawian children.

    Original languageEnglish
    Article number37
    Pages (from-to)37
    Number of pages9
    JournalMicrobiome
    Volume3
    DOIs
    Publication statusPublished - 2015

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