The effect of donor age on spontaneous and induced micronuclei

Michael Fenech, Alexander A. Morley

    Research output: Contribution to journalArticlepeer-review

    146 Citations (Scopus)

    Abstract

    The spontaneous micronucleus yield in lymphocyte cultures from healthy donors aged 0-82 years was estimated at 72 h and 96 h of culture. At both 72 and 96 h there was a positive correlation of micronucleus expression with increasing age (p < 0.001), with an approximately 4-fold increase in micronuclei in cultures from 80-year-old donors when compared to cultures from newborn donors. Since there is some evidence that the effect of DNA-damaging agents may increase with age, lymphocytes from individuals of various ages were exposed to X-rays and mitomycin C and micronuclei were scored after 72 and 96 h of culture. Micronucleus formation after exposure to these agents was, however, decreased in cells from elderly individuals, most likely due to kinetic differences between the lymphocytes of old and young individuals.

    Original languageEnglish
    Pages (from-to)99-105
    Number of pages7
    JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
    Volume148
    Issue number1-2
    DOIs
    Publication statusPublished - Feb 1985

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