The effects of exogenous additives on the vitality of Chinese pearl oyster (Pinctada fucata) sperm under different conditions

During the artificial breeding process of Chinese pearl oysters, the quality and vitality of sperm and eggs are pivotal in determining the success rate of fertilization. This study delves into the impact of externally added fructose, glucose, and ammoniated water on sperm vitality and lifespan under varying temperature conditions. The results indicate that the addition of exogenous energy sources and cell protectants, specifically monosaccharide energy substances, significantly improves sperm vitality. Moreover, under 4°C conditions, adding 2.8 mmol/L fructose, glucose, and 2 mmol/L ammoniated water to sperm samples significantly enhances sperm vitality and prolongs their lifespan. This provides strong evidence for the feasibility of short-term preservation and vitality enhancement strategies for Chinese pearl oyster sperm and also offers important scientific support for optimizing the artificial reproduction techniques of Chinese pearl oysters.