The Effects of Vibration Frequency on Oxidative Stress, Digestive Enzymes and ATPases of Crimson Snapper (Lutjanus erythropterus) Fry during Transport

In this study, we sought to characterize the effect of water vibration frequency stress on crimson snapper (Lutjanus erythropterus) survival to determine an optimal transportation speed. To achieve this, we used a transport tank (25 cm × 17 cm × 16 cm) to simulate the transport process. After 8 h at five different vibration frequencies (D1 = 75 rpm, D2 = 105 rpm, D3 = 135 rpm, D4 = 165 rpm, and D5 = 195 rpm), the pH and dissolved oxygen (DO) levels in the tanks decreased; ammonia nitrogen levels (NH₄-N) and temperature (T) increased with increasing density; and significant changes in oxidative stress biomarkers, digestive enzymes, and ATPase levels were observed in crimson snapper fry. The enzyme activity increased and reached the maximum value at 195 rpm. The experimental results suggested that during the actual transport, when using transport tanks, the length of the transport time was less than 8 h, and setting the vibration frequency for transportation at 135 rpm was more appropriate, that is, a speed of 50 km/h for transporting crimson snapper fry.