The end of bad hair days

Oliva Handt, Adrian Linacre

Research output: Contribution to conferencePaper

6 Citations (Scopus)

Abstract

DNA typing of a single hair using conventional DNA extraction processes can often result in no DNA profile but here we describe augmenting the direct-PCR approach resulting in an increased number of alleles being generated. The process includes the use of Prep-n-Go™ buffer prior to direct PCR using the recently released GlobalFiler® STR kit. Up-loadable DNA profiles were generated from all anagen hairs tested. Telogen hairs using Prep-n-Go™ resulted in 36% of the hairs generating up-loadable DNA profiles; this compared with 25% if no Pre-n-Go™ was used in the direct PCR. The addition of this step to the direct PCR of single hairs, both anagen and telogen, resulted in improved success in generating alleles using GlobalFiler®.

Original languageEnglish
Pagese146-e148
DOIs
Publication statusPublished - 2015
Event26th International Society for Forensic Genetics Congress - Krakow, Poland
Duration: 31 Aug 20155 Sep 2015

Conference

Conference26th International Society for Forensic Genetics Congress
CountryPoland
CityKrakow
Period31/08/155/09/15

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