The formation of proximate carcinogens from three polycyclic aromatic compounds by human liver microsomes

Sugiyanto; C. E. Scharping; M. E. Mcmanus; D. J. Birkett; G. M. Holder; A. J. Ryan

doi:10.3109/00498259209053158

The formation of proximate carcinogens from three polycyclic aromatic compounds by human liver microsomes

Sugiyanto, C. E. Scharping, M. E. Mcmanus, D. J. Birkett, G. M. Holder, A. J. Ryan

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

1. The metabolism of ³H-benzo[a]pyrene (BP), ³H-7-methylbenz[c]acridine (7MBAC) and ³H-dibenz[a, j]acridine (DBAJAC) have been studied in human liver microsomes from 13 subjects. 2. When the metabolism of these carcinogens to more polar ethyl acetate-soluble metabolites were compared, the activities towards the nitrogenous careinogens were twice that determined for BP. 3. The specific rates of formation of the three proximate carcinogens, BP-7,8-dihydrodiol, 7MBAC-3,4-dihydrodiol and DBAJAC-3,4-dihydrodiol per nmol cytochrome P-450 for 12 subjects were positively correlated. 4. These dihydrodiols constituted 5.9±0.7%mean±SEM), 57.8±2.6% and 3.0±0.4% of the total metabolites identified by cochromatography with standards, 7MBAC, DBAJAC and BP respectively.

Original language	English
Pages (from-to)	1299-1307
Number of pages	9
Journal	Xenobiotica
Volume	22
Issue number	11
DOIs	https://doi.org/10.3109/00498259209053158
Publication status	Published - 1992

Access to Document

10.3109/00498259209053158Licence: In Copyright

Cite this

@article{df1eadfc57bd43b18902aec41ea078c2,

title = "The formation of proximate carcinogens from three polycyclic aromatic compounds by human liver microsomes",

abstract = "1. The metabolism of 3H-benzo[a]pyrene (BP), 3H-7-methylbenz[c]acridine (7MBAC) and 3H-dibenz[a, j]acridine (DBAJAC) have been studied in human liver microsomes from 13 subjects. 2. When the metabolism of these carcinogens to more polar ethyl acetate-soluble metabolites were compared, the activities towards the nitrogenous careinogens were twice that determined for BP. 3. The specific rates of formation of the three proximate carcinogens, BP-7,8-dihydrodiol, 7MBAC-3,4-dihydrodiol and DBAJAC-3,4-dihydrodiol per nmol cytochrome P-450 for 12 subjects were positively correlated. 4. These dihydrodiols constituted 5.9±0.7%mean±SEM), 57.8±2.6% and 3.0±0.4% of the total metabolites identified by cochromatography with standards, 7MBAC, DBAJAC and BP respectively.",

author = "Sugiyanto and Scharping, {C. E.} and Mcmanus, {M. E.} and Birkett, {D. J.} and Holder, {G. M.} and Ryan, {A. J.}",

year = "1992",

doi = "10.3109/00498259209053158",

language = "English",

volume = "22",

pages = "1299--1307",

journal = "Xenobiotica",

issn = "0049-8254",

publisher = "Taylor & Francis",

number = "11",

}

TY - JOUR

T1 - The formation of proximate carcinogens from three polycyclic aromatic compounds by human liver microsomes

AU - Sugiyanto,

AU - Scharping, C. E.

AU - Mcmanus, M. E.

AU - Birkett, D. J.

AU - Holder, G. M.

AU - Ryan, A. J.

PY - 1992

Y1 - 1992

N2 - 1. The metabolism of 3H-benzo[a]pyrene (BP), 3H-7-methylbenz[c]acridine (7MBAC) and 3H-dibenz[a, j]acridine (DBAJAC) have been studied in human liver microsomes from 13 subjects. 2. When the metabolism of these carcinogens to more polar ethyl acetate-soluble metabolites were compared, the activities towards the nitrogenous careinogens were twice that determined for BP. 3. The specific rates of formation of the three proximate carcinogens, BP-7,8-dihydrodiol, 7MBAC-3,4-dihydrodiol and DBAJAC-3,4-dihydrodiol per nmol cytochrome P-450 for 12 subjects were positively correlated. 4. These dihydrodiols constituted 5.9±0.7%mean±SEM), 57.8±2.6% and 3.0±0.4% of the total metabolites identified by cochromatography with standards, 7MBAC, DBAJAC and BP respectively.

AB - 1. The metabolism of 3H-benzo[a]pyrene (BP), 3H-7-methylbenz[c]acridine (7MBAC) and 3H-dibenz[a, j]acridine (DBAJAC) have been studied in human liver microsomes from 13 subjects. 2. When the metabolism of these carcinogens to more polar ethyl acetate-soluble metabolites were compared, the activities towards the nitrogenous careinogens were twice that determined for BP. 3. The specific rates of formation of the three proximate carcinogens, BP-7,8-dihydrodiol, 7MBAC-3,4-dihydrodiol and DBAJAC-3,4-dihydrodiol per nmol cytochrome P-450 for 12 subjects were positively correlated. 4. These dihydrodiols constituted 5.9±0.7%mean±SEM), 57.8±2.6% and 3.0±0.4% of the total metabolites identified by cochromatography with standards, 7MBAC, DBAJAC and BP respectively.

UR - http://www.scopus.com/inward/record.url?scp=0026591986&partnerID=8YFLogxK

U2 - 10.3109/00498259209053158

DO - 10.3109/00498259209053158

M3 - Article

C2 - 1492422

AN - SCOPUS:0026591986

SN - 0049-8254

VL - 22

SP - 1299

EP - 1307

JO - Xenobiotica

JF - Xenobiotica

IS - 11

ER -

The formation of proximate carcinogens from three polycyclic aromatic compounds by human liver microsomes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this