TY - JOUR
T1 - The general transcription repressor TaDr1 is co-expressed with TaVrn1 and TaFT1 in bread wheat under drought
AU - Zotova, Lyudmila
AU - Kurishbayev, Akhylbek
AU - Jatayev, Satyvaldy
AU - Goncharov, Nikolay P.
AU - Shamambayeva, Nazgul
AU - Kashapov, Azamat
AU - Nuralov, Arystan
AU - Otemissova, Ainur
AU - Sereda, Sergey
AU - Shvidchenko, Vladimir
AU - Lopato, Sergiy
AU - Schramm, Carly
AU - Jenkins, Colin
AU - Soole, Kathleen
AU - Langridge, Peter
AU - Shavrukov, Yuri
PY - 2019/2/8
Y1 - 2019/2/8
N2 - The general transcription repressor, TaDr1 gene, was identified during screening of a wheat SNP database using the Amplifluor-like SNP marker KATU-W62. Together with two genes described earlier, TaDr1A and TaDr1B, they represent a set of three homeologous genes in the wheat genome. Under drought, the total expression profiles of all three genes varied between different bread wheat cultivars. Plants of four high-yielding cultivars exposed to drought showed a 2.0-2.4-fold increase in TaDr1 expression compared to controls. Less strong, but significant 1.3-1.8-fold up-regulation of the TaDr1 transcript levels was observed in four low-yielding cultivars. TaVrn1 and TaFT1, which controls the transition to flowering, revealed similar profiles of expression as TaDr1. Expression levels of all three genes were in good correlation with grain yields of evaluated cultivars growing in the field under water-limited conditions. The results could indicate the involvement of all three genes in the same regulatory pathway, where the general transcription repressor TaDr1 may control expression of TaVrn1 and TaFT1 and, consequently, flowering time. The strength of these genes expression can lead to phenological changes that affect plant productivity and hence explain differences in the adaptation of the examined wheat cultivars to the dry environment of Northern and Central Kazakhstan. The Amplifluor-like SNP marker KATU-W62 used in this work can be applied to the identification of wheat cultivars differing in alleles at the TaDr1 locus and in screening hybrids.
AB - The general transcription repressor, TaDr1 gene, was identified during screening of a wheat SNP database using the Amplifluor-like SNP marker KATU-W62. Together with two genes described earlier, TaDr1A and TaDr1B, they represent a set of three homeologous genes in the wheat genome. Under drought, the total expression profiles of all three genes varied between different bread wheat cultivars. Plants of four high-yielding cultivars exposed to drought showed a 2.0-2.4-fold increase in TaDr1 expression compared to controls. Less strong, but significant 1.3-1.8-fold up-regulation of the TaDr1 transcript levels was observed in four low-yielding cultivars. TaVrn1 and TaFT1, which controls the transition to flowering, revealed similar profiles of expression as TaDr1. Expression levels of all three genes were in good correlation with grain yields of evaluated cultivars growing in the field under water-limited conditions. The results could indicate the involvement of all three genes in the same regulatory pathway, where the general transcription repressor TaDr1 may control expression of TaVrn1 and TaFT1 and, consequently, flowering time. The strength of these genes expression can lead to phenological changes that affect plant productivity and hence explain differences in the adaptation of the examined wheat cultivars to the dry environment of Northern and Central Kazakhstan. The Amplifluor-like SNP marker KATU-W62 used in this work can be applied to the identification of wheat cultivars differing in alleles at the TaDr1 locus and in screening hybrids.
KW - Amplifluor-like SNP marker
KW - Bioinformatics
KW - Drought
KW - General repressor of transcription
KW - TaDr1
KW - TaFT1
KW - TaVrn1
UR - http://www.scopus.com/inward/record.url?scp=85062622895&partnerID=8YFLogxK
U2 - 10.3389/fgene.2019.00063
DO - 10.3389/fgene.2019.00063
M3 - Article
AN - SCOPUS:85062622895
VL - 10
JO - Frontiers in Genetics
JF - Frontiers in Genetics
SN - 1664-8021
M1 - 63
ER -