The HLA-A mutation assay: Improved technique and normal results

M. A. McCarron; A. Kutlaca; A. A. Morley

doi:10.1016/0165-7992(89)90118-8

The HLA-A mutation assay: Improved technique and normal results

M. A. McCarron, A. Kutlaca, A. A. Morley

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The HLA-A assay for detection of mutant lymphocytes has been modified, principally by incorporation of an overnight-incubation step which has resulted in improved immunoselection. For 83 estimations on cells from 21 normal individuals with a mean age of 35, the in vivo mutant frequency was 2.99 × 10^-5 ± 1.48 × 10^-5 (mean ± 1 SD). For 11 estimations on cells from 10 elderly individuals with a mean age of 78, the mutant frequency was significantly greater, being 7.16 × 10^-5 ± 4.06 × 10^-5. Similar results were obtained when HLA-A2 or HLA-A3 mutants were enumerated. Mutant frequency measured at the HLA-A locus is almost an order of magnitude greater than that measured at the hypoxanthine phosphoribosyl transferase locus, but at least the major part of this difference is likely to be due to factors other than a difference in genetic stability between the two loci.

Original language	English
Pages (from-to)	189-193
Number of pages	5
Journal	Mutation Research Letters
Volume	225
Issue number	4
DOIs	https://doi.org/10.1016/0165-7992(89)90118-8
Publication status	Published - Apr 1989
Externally published	Yes

Keywords

HLA
HPRT
Hypoxanthine phosphoribosyl transferase
Lymphocytes

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title = "The HLA-A mutation assay: Improved technique and normal results",

abstract = "The HLA-A assay for detection of mutant lymphocytes has been modified, principally by incorporation of an overnight-incubation step which has resulted in improved immunoselection. For 83 estimations on cells from 21 normal individuals with a mean age of 35, the in vivo mutant frequency was 2.99 × 10-5 ± 1.48 × 10-5 (mean ± 1 SD). For 11 estimations on cells from 10 elderly individuals with a mean age of 78, the mutant frequency was significantly greater, being 7.16 × 10-5 ± 4.06 × 10-5. Similar results were obtained when HLA-A2 or HLA-A3 mutants were enumerated. Mutant frequency measured at the HLA-A locus is almost an order of magnitude greater than that measured at the hypoxanthine phosphoribosyl transferase locus, but at least the major part of this difference is likely to be due to factors other than a difference in genetic stability between the two loci.",

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T2 - Improved technique and normal results

AU - McCarron, M. A.

AU - Kutlaca, A.

AU - Morley, A. A.

PY - 1989/4

Y1 - 1989/4

N2 - The HLA-A assay for detection of mutant lymphocytes has been modified, principally by incorporation of an overnight-incubation step which has resulted in improved immunoselection. For 83 estimations on cells from 21 normal individuals with a mean age of 35, the in vivo mutant frequency was 2.99 × 10-5 ± 1.48 × 10-5 (mean ± 1 SD). For 11 estimations on cells from 10 elderly individuals with a mean age of 78, the mutant frequency was significantly greater, being 7.16 × 10-5 ± 4.06 × 10-5. Similar results were obtained when HLA-A2 or HLA-A3 mutants were enumerated. Mutant frequency measured at the HLA-A locus is almost an order of magnitude greater than that measured at the hypoxanthine phosphoribosyl transferase locus, but at least the major part of this difference is likely to be due to factors other than a difference in genetic stability between the two loci.

AB - The HLA-A assay for detection of mutant lymphocytes has been modified, principally by incorporation of an overnight-incubation step which has resulted in improved immunoselection. For 83 estimations on cells from 21 normal individuals with a mean age of 35, the in vivo mutant frequency was 2.99 × 10-5 ± 1.48 × 10-5 (mean ± 1 SD). For 11 estimations on cells from 10 elderly individuals with a mean age of 78, the mutant frequency was significantly greater, being 7.16 × 10-5 ± 4.06 × 10-5. Similar results were obtained when HLA-A2 or HLA-A3 mutants were enumerated. Mutant frequency measured at the HLA-A locus is almost an order of magnitude greater than that measured at the hypoxanthine phosphoribosyl transferase locus, but at least the major part of this difference is likely to be due to factors other than a difference in genetic stability between the two loci.

KW - HLA

KW - HPRT

KW - Hypoxanthine phosphoribosyl transferase

KW - Lymphocytes

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The HLA-A mutation assay: Improved technique and normal results

Abstract

Keywords

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