The identification, cloning and functional expression of the gene encoding orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum.

R.I. Menz, O Cinquin, R Christopherson

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    The coding region of a putative orotidine 5'-monophosphate decarboxylase gene from Plasmodium falciparum was identified in genomic data from the Malarial Genome Sequencing Project. The gene encodes a protein of 323 amino acids with a predicted molecular weight of 37.8 kDa. The gene was cloned into a bacterial expression vector and over-expressed in Escherichia coli. The recombinant protein was purified and shown to have orotidine 5'-monophosphate decarboxylase activity, confirming the identity of the gene.
    Original languageEnglish
    Pages (from-to)469-476
    Number of pages8
    JournalAnnals of Tropical Medicine and Parasitology
    Volume96
    Issue number5
    DOIs
    Publication statusPublished - 2002

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