THE INFLUENCE OF AGE OF TEMPLATE DNA DERIVED FROM ARCHIVAL TISSUE ON THE OUTCOME OF THE POLYMERASE CHAIN REACTION

P. J. Hewett; F. Firgaira; A. Morley

doi:10.1111/j.1445-2197.1994.tb02286.x

THE INFLUENCE OF AGE OF TEMPLATE DNA DERIVED FROM ARCHIVAL TISSUE ON THE OUTCOME OF THE POLYMERASE CHAIN REACTION

P. J. Hewett, F. Firgaira, A. Morley

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The analysis of DNA from archival tumour tissue for molecular alterations has been facilitated by the use of the polymerase chain reaction (PCR). Degradation of tissue prior to fixation and the nature of the fixative used influence successful amplification from archival tissue. Age of the archival tissue may also be a factor. To determine if this was so, DNA was extracted from 30 archival specimens of spleen spanning a 15 year period. Polymerase chain reaction was performed on all specimens using primers for exon 2 (307 bp) and exon 9 (1278 bp) of the hypoxanthine phosphoribosyl transferase (HPRT) gene. It was not possible to show that the age of archival tissue had an influence on the capacity to amplify exon 2 of the HPRT gene. It was not possible to amplify exon 9 of the HPRT gene from archival tissue.

Original language	English
Pages (from-to)	558-559
Number of pages	2
Journal	Australian and New Zealand Journal of Surgery
Volume	64
Issue number	8
DOIs	https://doi.org/10.1111/j.1445-2197.1994.tb02286.x
Publication status	Published - Aug 1994
Externally published	Yes

Keywords

age
archival tissue
polymerase chain reaction

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title = "THE INFLUENCE OF AGE OF TEMPLATE DNA DERIVED FROM ARCHIVAL TISSUE ON THE OUTCOME OF THE POLYMERASE CHAIN REACTION",

abstract = "The analysis of DNA from archival tumour tissue for molecular alterations has been facilitated by the use of the polymerase chain reaction (PCR). Degradation of tissue prior to fixation and the nature of the fixative used influence successful amplification from archival tissue. Age of the archival tissue may also be a factor. To determine if this was so, DNA was extracted from 30 archival specimens of spleen spanning a 15 year period. Polymerase chain reaction was performed on all specimens using primers for exon 2 (307 bp) and exon 9 (1278 bp) of the hypoxanthine phosphoribosyl transferase (HPRT) gene. It was not possible to show that the age of archival tissue had an influence on the capacity to amplify exon 2 of the HPRT gene. It was not possible to amplify exon 9 of the HPRT gene from archival tissue.",

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THE INFLUENCE OF AGE OF TEMPLATE DNA DERIVED FROM ARCHIVAL TISSUE ON THE OUTCOME OF THE POLYMERASE CHAIN REACTION

Abstract

Keywords

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