TY - JOUR
T1 - The Interplay between Surface Nanotopography and Chemistry Modulates Collagen i and III Deposition by Human Dermal Fibroblasts
AU - Bachhuka, Akash
AU - Hayball, John Dominic
AU - Smith, Louise E.
AU - Vasilev, Krasimir
PY - 2017/2/22
Y1 - 2017/2/22
N2 - The events within the foreign body response are similar to, but ultimately different than, the wound healing cascade. Collagen production by fibroblasts is known to play a vital role in wound healing and device fibrous encapsulation. However, the influence of surface nanotopography on collagen deposition by these cells has not been reported so far. To address this gap, we have developed model substrata having surface nanotopography of controlled height of 16, 38, and 68 nm and tailored outermost surface chemistry of amines, carboxyl acid, and pure hydrocarbon. Fibroblast adhesion was reduced on nanotopographically modified surfaces compared to the smooth control. Furthermore, amine and acid functionalized surfaces showed increased cell proliferation over hydrophobic hydrocarbon surfaces. Collagen III production increased from day 3 to day 8 and then decreased from day 8 to day 16 on all surfaces, while collagen I deposition increased throughout the duration of 16 days. Our data show that the initial collagen I and III deposition can be modulated by selecting desired combinations of surface nanotopography and chemistry. This study provides useful knowledge that could help in tuning fibrous capsule formation and in turn govern the fate of implantable biomaterial devices.
AB - The events within the foreign body response are similar to, but ultimately different than, the wound healing cascade. Collagen production by fibroblasts is known to play a vital role in wound healing and device fibrous encapsulation. However, the influence of surface nanotopography on collagen deposition by these cells has not been reported so far. To address this gap, we have developed model substrata having surface nanotopography of controlled height of 16, 38, and 68 nm and tailored outermost surface chemistry of amines, carboxyl acid, and pure hydrocarbon. Fibroblast adhesion was reduced on nanotopographically modified surfaces compared to the smooth control. Furthermore, amine and acid functionalized surfaces showed increased cell proliferation over hydrophobic hydrocarbon surfaces. Collagen III production increased from day 3 to day 8 and then decreased from day 8 to day 16 on all surfaces, while collagen I deposition increased throughout the duration of 16 days. Our data show that the initial collagen I and III deposition can be modulated by selecting desired combinations of surface nanotopography and chemistry. This study provides useful knowledge that could help in tuning fibrous capsule formation and in turn govern the fate of implantable biomaterial devices.
KW - biomaterials
KW - collagens I and III
KW - foreign body response
KW - plasma polymerization
KW - surface nanotopography
UR - http://www.scopus.com/inward/record.url?scp=85013374271&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/DP15104212
UR - http://purl.org/au-research/grants/NHMRC/1122825
U2 - 10.1021/acsami.6b15932
DO - 10.1021/acsami.6b15932
M3 - Article
C2 - 28156094
AN - SCOPUS:85013374271
SN - 1944-8244
VL - 9
SP - 5874
EP - 5884
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 7
ER -