The isolation and characterisation of a gene encoding superoxide dismutase from Bradyrhizobium sp. (Parasponia) strain ANU289

P. W. Thygesen, J. Whelan, M. K. Morell, D. A. Day

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Abstract

Bradyrhizobium sp. (Parasponia) strain ANU289 expresses a single Mn-SOD in both the vegetative and symbiotic states. A 500 bp sod-homologous sequence was amplified from genomic DNA of strain ANU289 using PCR. A 1.3 kb SalI fragment was subsequently cloned which contained an ORF, sodA, encoding a 23 Kd protein. This putative SOD shares considerable homology with other Mn-SODs and analysis of the sodA sequence predicts that it is expressed. A lacZ-sodA fusion complemented the SOD-deficiency of E. coli QC779 and resulted in the expression of SOD activity in both mutant and wild type E. coli. We conclude that sodA encodes the Mn-SOD of strain ANU289.

Original languageEnglish
Pages (from-to)401-412
Number of pages12
JournalBiochemistry and Molecular Biology International
Volume37
Issue number3
DOIs
Publication statusPublished - 1 Dec 1995
Externally publishedYes

Keywords

  • Bradyrhizobium
  • E. coli QC779
  • Mn-SOD
  • Parasponia
  • SOD
  • sodA
  • Strain ANU289
  • Superoxide dismutase

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