The Nasal Innate Immune Proteome After Saline Irrigation: A Pilot Study in Healthy Individuals

Emily A. Harcourt-Smith; Emerson T. Krstic; Belinda J. Soekov-Pearce; Alex D. Colella; Nusha Chegeni; Timothy K. Chataway; Charmaine M. Woods; Kamelya Aliakbari; A. Simon Carney

doi:10.1177/19458924231159176

The Nasal Innate Immune Proteome After Saline Irrigation: A Pilot Study in Healthy Individuals

Emily A. Harcourt-Smith, Emerson T. Krstic, Belinda J. Soekov-Pearce, Alex D. Colella, Nusha Chegeni, Timothy K. Chataway, Charmaine M. Woods, Kamelya Aliakbari, A. Simon Carney

College of Medicine and Public Health

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

50 Downloads (Pure)

Abstract

Background: Previous research has shown diminished nasal immune function following nasal saline irrigation (NSI), returning to baseline at 6 hours. The aim of this study was to examine the immune nasal proteome before and after 14 days of nasal irrigation.

Methods: Seventeen healthy volunteers received either isotonic (IsoSal) or low salt (LowNa) NSI. Nasal secretions were collected before and 30 min after NSI at baseline and again after 14 days. Specimens were analyzed using mass spectrometry to detect proteins of relevance to nasal immune function.

Results: One thousand eight hundred and sixty-five proteins were identified with significant changes in 71 proteins, of which 23 were identified as part of the innate immune system. Baseline analysis demonstrated an increase of 9 innate proteins after NSI, most after IsoSal. After 14 days, a greater increase in innate peptides was present, with most now in the LowNa group. When NSI solutions were compared, a significant increase in 4 innate proteins, including a 211% in lysozyme, was detected in the LowNa group.

Conclusion: LowNa NSI demonstrates evidence of improving the innate immune secretions, especially lysozyme, in healthy volunteers.

Original language	English
Pages (from-to)	419-428
Number of pages	10
Journal	American Journal of Rhinology and Allergy
Volume	37
Issue number	4
Early online date	27 Feb 2023
DOIs	https://doi.org/10.1177/19458924231159176
Publication status	Published - Jul 2023

Keywords

innate immunity
intranasal administration
lactoferrin
lysozyme
mass spectrometry
nasal mucosa
proteomics
rhinitis
saline solution
sinusitis

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10.1177/19458924231159176Licence: CC BY-NC

Final published versionFinal published version, 430 KBLicence: CC BY-NC

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title = "The Nasal Innate Immune Proteome After Saline Irrigation: A Pilot Study in Healthy Individuals",

abstract = "Background: Previous research has shown diminished nasal immune function following nasal saline irrigation (NSI), returning to baseline at 6 hours. The aim of this study was to examine the immune nasal proteome before and after 14 days of nasal irrigation. Methods: Seventeen healthy volunteers received either isotonic (IsoSal) or low salt (LowNa) NSI. Nasal secretions were collected before and 30 min after NSI at baseline and again after 14 days. Specimens were analyzed using mass spectrometry to detect proteins of relevance to nasal immune function. Results: One thousand eight hundred and sixty-five proteins were identified with significant changes in 71 proteins, of which 23 were identified as part of the innate immune system. Baseline analysis demonstrated an increase of 9 innate proteins after NSI, most after IsoSal. After 14 days, a greater increase in innate peptides was present, with most now in the LowNa group. When NSI solutions were compared, a significant increase in 4 innate proteins, including a 211% in lysozyme, was detected in the LowNa group. Conclusion: LowNa NSI demonstrates evidence of improving the innate immune secretions, especially lysozyme, in healthy volunteers.",

keywords = "innate immunity, intranasal administration, lactoferrin, lysozyme, mass spectrometry, nasal mucosa, proteomics, rhinitis, saline solution, sinusitis",

author = "Harcourt-Smith, {Emily A.} and Krstic, {Emerson T.} and Soekov-Pearce, {Belinda J.} and Colella, {Alex D.} and Nusha Chegeni and Chataway, {Timothy K.} and Woods, {Charmaine M.} and Kamelya Aliakbari and Carney, {A. Simon}",

year = "2023",

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doi = "10.1177/19458924231159176",

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T1 - The Nasal Innate Immune Proteome After Saline Irrigation

T2 - A Pilot Study in Healthy Individuals

AU - Harcourt-Smith, Emily A.

AU - Krstic, Emerson T.

AU - Soekov-Pearce, Belinda J.

AU - Colella, Alex D.

AU - Chegeni, Nusha

AU - Chataway, Timothy K.

AU - Woods, Charmaine M.

AU - Aliakbari, Kamelya

AU - Carney, A. Simon

PY - 2023/7

Y1 - 2023/7

N2 - Background: Previous research has shown diminished nasal immune function following nasal saline irrigation (NSI), returning to baseline at 6 hours. The aim of this study was to examine the immune nasal proteome before and after 14 days of nasal irrigation. Methods: Seventeen healthy volunteers received either isotonic (IsoSal) or low salt (LowNa) NSI. Nasal secretions were collected before and 30 min after NSI at baseline and again after 14 days. Specimens were analyzed using mass spectrometry to detect proteins of relevance to nasal immune function. Results: One thousand eight hundred and sixty-five proteins were identified with significant changes in 71 proteins, of which 23 were identified as part of the innate immune system. Baseline analysis demonstrated an increase of 9 innate proteins after NSI, most after IsoSal. After 14 days, a greater increase in innate peptides was present, with most now in the LowNa group. When NSI solutions were compared, a significant increase in 4 innate proteins, including a 211% in lysozyme, was detected in the LowNa group. Conclusion: LowNa NSI demonstrates evidence of improving the innate immune secretions, especially lysozyme, in healthy volunteers.

AB - Background: Previous research has shown diminished nasal immune function following nasal saline irrigation (NSI), returning to baseline at 6 hours. The aim of this study was to examine the immune nasal proteome before and after 14 days of nasal irrigation. Methods: Seventeen healthy volunteers received either isotonic (IsoSal) or low salt (LowNa) NSI. Nasal secretions were collected before and 30 min after NSI at baseline and again after 14 days. Specimens were analyzed using mass spectrometry to detect proteins of relevance to nasal immune function. Results: One thousand eight hundred and sixty-five proteins were identified with significant changes in 71 proteins, of which 23 were identified as part of the innate immune system. Baseline analysis demonstrated an increase of 9 innate proteins after NSI, most after IsoSal. After 14 days, a greater increase in innate peptides was present, with most now in the LowNa group. When NSI solutions were compared, a significant increase in 4 innate proteins, including a 211% in lysozyme, was detected in the LowNa group. Conclusion: LowNa NSI demonstrates evidence of improving the innate immune secretions, especially lysozyme, in healthy volunteers.

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KW - lysozyme

KW - mass spectrometry

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KW - rhinitis

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KW - sinusitis

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The Nasal Innate Immune Proteome After Saline Irrigation: A Pilot Study in Healthy Individuals

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