The neuro-protective role of Sir2 in the process of neuro-degeneration of the SCA3/MJD model flies is dependent on autophagy function

Ai Yeng Zeng; Jing Lei Zhu; Kang Kang Hong; Zhuohua Zhang; Ran Duan; Li Sun; Cheng Wei Liu; Xiao Li Wei; Li Li Wei; Mei Ling Chen; Xiao Hui Lin; Wei Chen; Qing Hua Li

doi:10.3724/SP.J.1206.2011.00367

The neuro-protective role of Sir2 in the process of neuro-degeneration of the SCA3/MJD model flies is dependent on autophagy function

Ai Yeng Zeng, Jing Lei Zhu, Kang Kang Hong, Zhuohua Zhang, Ran Duan, Li Sun, Cheng Wei Liu, Xiao Li Wei, Li Li Wei, Mei Ling Chen, Xiao Hui Lin, Wei Chen, Qing Hua Li

Research output: Contribution to journal › Article › peer-review

Abstract

To confer the influence of Sir2 on pathogenesis of SCA3/MJD. GMR-GAL4 and Nrv2-GAL4 system SCA3/MJD transgenic Drosophila models were constructed by using the promoter GMR-GAL4 and Nrv2-GAL4 which drive target selective gene expression in cells of the developing eyes and motor neurons, respectively. Then, Sir2 protein was overexpressed in SCA3/MJD transgenic Drosophila models by genetic methods with or without in a background of RNAi knockdown of Atg7. Overexpression of endogenous Drosophila Sir2 not only notably suppresses the neurotoxicity of MJDtr-Q78 protein, but also significantly improves the movement ability of flies. Moreover, RNAi knockdown of Atg7 significantly Sir2's protection against SCA3/MJD Drosophila. We confirmed that overexpression of Sir2 could protect SCA3/MJD Drosophila models, and the protection role of Sir2 on SCA3/MJD Drosophila models is autophagy- dependent.

Original language	English
Pages (from-to)	250-256
Number of pages	7
Journal	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
Volume	39
Issue number	3
DOIs	https://doi.org/10.3724/SP.J.1206.2011.00367
Publication status	Published - 2012

Keywords

Ataxin-3
Neuroprotection
SCA3
Sir2
Transgenic Drosophila models

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Zeng, A. Y., Zhu, J. L., Hong, K. K., Zhang, Z., Duan, R., Sun, L., Liu, C. W., Wei, X. L., Wei, L. L., Chen, M. L., Lin, X. H., Chen, W., & Li, Q. H. (2012). The neuro-protective role of Sir2 in the process of neuro-degeneration of the SCA3/MJD model flies is dependent on autophagy function. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 39(3), 250-256. https://doi.org/10.3724/SP.J.1206.2011.00367

@article{3bbda167b3ce41a8b566c40040379b51,

title = "The neuro-protective role of Sir2 in the process of neuro-degeneration of the SCA3/MJD model flies is dependent on autophagy function",

abstract = "To confer the influence of Sir2 on pathogenesis of SCA3/MJD. GMR-GAL4 and Nrv2-GAL4 system SCA3/MJD transgenic Drosophila models were constructed by using the promoter GMR-GAL4 and Nrv2-GAL4 which drive target selective gene expression in cells of the developing eyes and motor neurons, respectively. Then, Sir2 protein was overexpressed in SCA3/MJD transgenic Drosophila models by genetic methods with or without in a background of RNAi knockdown of Atg7. Overexpression of endogenous Drosophila Sir2 not only notably suppresses the neurotoxicity of MJDtr-Q78 protein, but also significantly improves the movement ability of flies. Moreover, RNAi knockdown of Atg7 significantly Sir2's protection against SCA3/MJD Drosophila. We confirmed that overexpression of Sir2 could protect SCA3/MJD Drosophila models, and the protection role of Sir2 on SCA3/MJD Drosophila models is autophagy- dependent.",

keywords = "Ataxin-3, Neuroprotection, SCA3, Sir2, Transgenic Drosophila models",

author = "Zeng, {Ai Yeng} and Zhu, {Jing Lei} and Hong, {Kang Kang} and Zhuohua Zhang and Ran Duan and Li Sun and Liu, {Cheng Wei} and Wei, {Xiao Li} and Wei, {Li Li} and Chen, {Mei Ling} and Lin, {Xiao Hui} and Wei Chen and Li, {Qing Hua}",

year = "2012",

doi = "10.3724/SP.J.1206.2011.00367",

language = "English",

volume = "39",

pages = "250--256",

journal = "PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS",

issn = "1000-3282",

publisher = "Science Press",

number = "3",

}

Zeng, AY, Zhu, JL, Hong, KK, Zhang, Z, Duan, R, Sun, L, Liu, CW, Wei, XL, Wei, LL, Chen, ML, Lin, XH, Chen, W & Li, QH 2012, 'The neuro-protective role of Sir2 in the process of neuro-degeneration of the SCA3/MJD model flies is dependent on autophagy function', PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, vol. 39, no. 3, pp. 250-256. https://doi.org/10.3724/SP.J.1206.2011.00367

TY - JOUR

T1 - The neuro-protective role of Sir2 in the process of neuro-degeneration of the SCA3/MJD model flies is dependent on autophagy function

AU - Zeng, Ai Yeng

AU - Zhu, Jing Lei

AU - Hong, Kang Kang

AU - Zhang, Zhuohua

AU - Duan, Ran

AU - Sun, Li

AU - Liu, Cheng Wei

AU - Wei, Xiao Li

AU - Wei, Li Li

AU - Chen, Mei Ling

AU - Lin, Xiao Hui

AU - Chen, Wei

AU - Li, Qing Hua

PY - 2012

Y1 - 2012

N2 - To confer the influence of Sir2 on pathogenesis of SCA3/MJD. GMR-GAL4 and Nrv2-GAL4 system SCA3/MJD transgenic Drosophila models were constructed by using the promoter GMR-GAL4 and Nrv2-GAL4 which drive target selective gene expression in cells of the developing eyes and motor neurons, respectively. Then, Sir2 protein was overexpressed in SCA3/MJD transgenic Drosophila models by genetic methods with or without in a background of RNAi knockdown of Atg7. Overexpression of endogenous Drosophila Sir2 not only notably suppresses the neurotoxicity of MJDtr-Q78 protein, but also significantly improves the movement ability of flies. Moreover, RNAi knockdown of Atg7 significantly Sir2's protection against SCA3/MJD Drosophila. We confirmed that overexpression of Sir2 could protect SCA3/MJD Drosophila models, and the protection role of Sir2 on SCA3/MJD Drosophila models is autophagy- dependent.

AB - To confer the influence of Sir2 on pathogenesis of SCA3/MJD. GMR-GAL4 and Nrv2-GAL4 system SCA3/MJD transgenic Drosophila models were constructed by using the promoter GMR-GAL4 and Nrv2-GAL4 which drive target selective gene expression in cells of the developing eyes and motor neurons, respectively. Then, Sir2 protein was overexpressed in SCA3/MJD transgenic Drosophila models by genetic methods with or without in a background of RNAi knockdown of Atg7. Overexpression of endogenous Drosophila Sir2 not only notably suppresses the neurotoxicity of MJDtr-Q78 protein, but also significantly improves the movement ability of flies. Moreover, RNAi knockdown of Atg7 significantly Sir2's protection against SCA3/MJD Drosophila. We confirmed that overexpression of Sir2 could protect SCA3/MJD Drosophila models, and the protection role of Sir2 on SCA3/MJD Drosophila models is autophagy- dependent.

KW - Ataxin-3

KW - Neuroprotection

KW - SCA3

KW - Sir2

KW - Transgenic Drosophila models

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U2 - 10.3724/SP.J.1206.2011.00367

DO - 10.3724/SP.J.1206.2011.00367

M3 - Article

SN - 1000-3282

VL - 39

SP - 250

EP - 256

JO - PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS

JF - PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS

IS - 3

ER -

The neuro-protective role of Sir2 in the process of neuro-degeneration of the SCA3/MJD model flies is dependent on autophagy function

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Keywords

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