Abstract
Introduction: Metrnl or also known as Meteorin-like and IL-41, is a novel protein that has been described as both a cytokine and myokine and is thought to be predominantly immunoregulatory in nature.[1] A recent report found that IL-41 mRNA was highly expressed in psoriatic skin, when compared to other inflammatory skin diseases.[2] We investigated IL-41 protein expression in synovial tissue in RA (Rheumatoid Arthritis), PsA (Psoriatic Arthritis) and OA (Osteoarthritis) patients. IL-41 production was also evaluated in vitro from healthy enthesis samples, as the enthesis represents the primary lesion in PsA. [3]
Methods: IL-41 was measured in synovial fluid from PsA, OA patients by ELISA. Synovial biopsies from the aforementioned disease groups were stained for IL-41 by immunohistochemistry (IHC). Healthy entheseal tissue was isolated and studied in vitro to identify possible cellular sources of IL-41.
Results: IL-41 is highly expressed in the synovial fluid and synovial tissue of PsA patients (median 8118 pg/ml) when compared to OA patients (median 4720 pg/ml). We found that entheseal stromal cells were the dominant producer of IL-41 from the enthesis. Moreover, stromal derived IL-41, could be further induced by the addition of psoriatic relevant cytokines such as IL-17 and TNF.
Conclusion: IL-41 is expressed in psoriatic arthritis synovium and is present and inducible at the enthesis. Its functional effect in psoriatic inflammation remains to be fully elucidated.
Methods: IL-41 was measured in synovial fluid from PsA, OA patients by ELISA. Synovial biopsies from the aforementioned disease groups were stained for IL-41 by immunohistochemistry (IHC). Healthy entheseal tissue was isolated and studied in vitro to identify possible cellular sources of IL-41.
Results: IL-41 is highly expressed in the synovial fluid and synovial tissue of PsA patients (median 8118 pg/ml) when compared to OA patients (median 4720 pg/ml). We found that entheseal stromal cells were the dominant producer of IL-41 from the enthesis. Moreover, stromal derived IL-41, could be further induced by the addition of psoriatic relevant cytokines such as IL-17 and TNF.
Conclusion: IL-41 is expressed in psoriatic arthritis synovium and is present and inducible at the enthesis. Its functional effect in psoriatic inflammation remains to be fully elucidated.
Original language | English |
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Pages | 160 |
Number of pages | 1 |
Publication status | Published - 2019 |
Event | 7th Annual Meeting of the International Cytokine and Interferon Society - Vienna Duration: 20 Oct 2019 → 23 Oct 2019 |
Conference
Conference | 7th Annual Meeting of the International Cytokine and Interferon Society |
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Period | 20/10/19 → 23/10/19 |
Keywords
- rheumatoid arthritis
- cytokine
- Metrnl