The possible re-emergence of LDH as a diagnostic tool

Mark Shephard

doi:10.1016/S0031-3025(16)40001-2

Abstract

Reactivity with α-ketobutyrate and the effect of heat inactivation were used to reassess the relative merits of HBD and LDH as diagnostic aids in liver and heart disease. Purified LD1 and LD5 both reacted with α-ketobutyrate over a wide range of temperature and substrate concentrations. No one set of conditions adequately discriminated between these isoenzymes. Electrophoretic studies demonstrated that heat inactivation at 60° resulted in minimal loss of cardiac isoenzyme (LD1–2) activity after 1 h, while eliminating LD3–5 activities within 30 min. Estimation of the percentage of the total LDH activity remaining after heat treatment revealed a clear demarcation between patients with liver and heart disease, whilst poorer discrimination was observed with HBD. The value of pre- and post-heat LDH estimations in the differential diagnosis of liver and heart disease was demonstrated in patients with minimally elevated liver and cardiac enzymes.

Original language	English
Pages (from-to)	331
Journal	Pathology
Volume	11
Issue number	2
DOIs	https://doi.org/10.1016/S0031-3025(16)40001-2
Publication status	Published - 1979
Event	17th Annual Conference of the AACB - Adelaide, Australia Duration: 1 Jan 1979 → 2 Jan 1979

Access to Document

10.1016/S0031-3025(16)40001-2Licence: In Copyright

Cite this

@article{dadddf5b2b7c4ac48ea7b6ebe185e1f8,

title = "The possible re-emergence of LDH as a diagnostic tool",

abstract = "Reactivity with α-ketobutyrate and the effect of heat inactivation were used to reassess the relative merits of HBD and LDH as diagnostic aids in liver and heart disease. Purified LD1 and LD5 both reacted with α-ketobutyrate over a wide range of temperature and substrate concentrations. No one set of conditions adequately discriminated between these isoenzymes. Electrophoretic studies demonstrated that heat inactivation at 60° resulted in minimal loss of cardiac isoenzyme (LD1–2) activity after 1 h, while eliminating LD3–5 activities within 30 min. Estimation of the percentage of the total LDH activity remaining after heat treatment revealed a clear demarcation between patients with liver and heart disease, whilst poorer discrimination was observed with HBD. The value of pre- and post-heat LDH estimations in the differential diagnosis of liver and heart disease was demonstrated in patients with minimally elevated liver and cardiac enzymes.",

author = "Mark Shephard",

year = "1979",

doi = "10.1016/S0031-3025(16)40001-2",

language = "English",

volume = "11",

pages = "331",

journal = "Pathology",

issn = "0031-3025",

publisher = "Elsevier Ltd.",

number = "2",

note = "17th Annual Conference of the AACB ; Conference date: 01-01-1979 Through 02-01-1979",

}

TY - JOUR

T1 - The possible re-emergence of LDH as a diagnostic tool

AU - Shephard, Mark

PY - 1979

Y1 - 1979

N2 - Reactivity with α-ketobutyrate and the effect of heat inactivation were used to reassess the relative merits of HBD and LDH as diagnostic aids in liver and heart disease. Purified LD1 and LD5 both reacted with α-ketobutyrate over a wide range of temperature and substrate concentrations. No one set of conditions adequately discriminated between these isoenzymes. Electrophoretic studies demonstrated that heat inactivation at 60° resulted in minimal loss of cardiac isoenzyme (LD1–2) activity after 1 h, while eliminating LD3–5 activities within 30 min. Estimation of the percentage of the total LDH activity remaining after heat treatment revealed a clear demarcation between patients with liver and heart disease, whilst poorer discrimination was observed with HBD. The value of pre- and post-heat LDH estimations in the differential diagnosis of liver and heart disease was demonstrated in patients with minimally elevated liver and cardiac enzymes.

AB - Reactivity with α-ketobutyrate and the effect of heat inactivation were used to reassess the relative merits of HBD and LDH as diagnostic aids in liver and heart disease. Purified LD1 and LD5 both reacted with α-ketobutyrate over a wide range of temperature and substrate concentrations. No one set of conditions adequately discriminated between these isoenzymes. Electrophoretic studies demonstrated that heat inactivation at 60° resulted in minimal loss of cardiac isoenzyme (LD1–2) activity after 1 h, while eliminating LD3–5 activities within 30 min. Estimation of the percentage of the total LDH activity remaining after heat treatment revealed a clear demarcation between patients with liver and heart disease, whilst poorer discrimination was observed with HBD. The value of pre- and post-heat LDH estimations in the differential diagnosis of liver and heart disease was demonstrated in patients with minimally elevated liver and cardiac enzymes.

U2 - 10.1016/S0031-3025(16)40001-2

DO - 10.1016/S0031-3025(16)40001-2

M3 - Meeting Abstract

SN - 0031-3025

VL - 11

SP - 331

JO - Pathology

JF - Pathology

IS - 2

T2 - 17th Annual Conference of the AACB

Y2 - 1 January 1979 through 2 January 1979

ER -

The possible re-emergence of LDH as a diagnostic tool

Abstract

Access to Document

Fingerprint

Cite this