The presence of antibodies to human dopamine-β-hydroxylase in commercially available antisera

Robert A. Rush; Sidney Udenfriend

doi:10.1016/0006-291X(74)91011-0

The presence of antibodies to human dopamine-β-hydroxylase in commercially available antisera

Robert A. Rush, Sidney Udenfriend

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Three criteria have been used to demonstrate the presence of antibodies to human dopamine-β-hydroxylase in commercially available antisera directed against various human serum fractions. These criteria are the inhibition of enzyme activity, complement fixation and binding of ¹²⁵I-labelled dopamine-β-hydroxylase to the immobilized antisera. The level of antibody present in some of these antisera was sufficient to allow their use in the radioimmunoassay of the enzyme. The possibility of other useful antibodies occurring in these and similar antisera is suggested.

Original language	English
Pages (from-to)	685-691
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(74)91011-0
Publication status	Published - 27 Nov 1974
Externally published	Yes

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title = "The presence of antibodies to human dopamine-β-hydroxylase in commercially available antisera",

abstract = "Three criteria have been used to demonstrate the presence of antibodies to human dopamine-β-hydroxylase in commercially available antisera directed against various human serum fractions. These criteria are the inhibition of enzyme activity, complement fixation and binding of 125I-labelled dopamine-β-hydroxylase to the immobilized antisera. The level of antibody present in some of these antisera was sufficient to allow their use in the radioimmunoassay of the enzyme. The possibility of other useful antibodies occurring in these and similar antisera is suggested.",

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AB - Three criteria have been used to demonstrate the presence of antibodies to human dopamine-β-hydroxylase in commercially available antisera directed against various human serum fractions. These criteria are the inhibition of enzyme activity, complement fixation and binding of 125I-labelled dopamine-β-hydroxylase to the immobilized antisera. The level of antibody present in some of these antisera was sufficient to allow their use in the radioimmunoassay of the enzyme. The possibility of other useful antibodies occurring in these and similar antisera is suggested.

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The presence of antibodies to human dopamine-β-hydroxylase in commercially available antisera

Abstract

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