The protective effects of XH-105 against radiation-induced intestinal injury

Ying Cheng, Yinping Dong, Qinlian Hou, Jing Wu, Wei Zhang, Hongqi Tian, Deguan Li

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Radiation-induced intestinal injury is one of the major side effects in patients receiving radiation therapy. There is no specific treatment for radiation enteritis in the clinic. We designed and synthesized a new compound named XH-105, which is expected to cleave into polyphenol and aminothiol in vivo to mitigate radiation injury. In the following study, we describe the beneficial effects of XH-105 against radiation-induced intestinal injury. C57BL/6J mice were treated by gavage with XH-105 1 hour before total body irradiation (TBI), and the survival rate was monitored. Histological changes were examined, and survival of Lgr5 + intestinal stem cells Ki67 + cells, villi + enterocytes and lysozymes was determined by immunohistochemistry. DNA damage and cellular apoptosis in intestinal tissue were also evaluated. Compared to vehicle-treated mice after TBI, XH-105 treatment significantly enhanced the survival rate, attenuated structural damage of the small intestine, decreased the apoptotic rate, reduced DNA damage, maintained cell regeneration and promoted crypt proliferation and differentiation. XH-105 also reduced the expression of Bax and p53 in the small intestine. These data suggest that XH-105 is beneficial for the protection of radiation-induced intestinal injury by inhibiting the p53-dependent apoptosis signalling pathway.

    Original languageEnglish
    Pages (from-to)2238-2247
    Number of pages10
    JournalJOURNAL OF CELLULAR AND MOLECULAR MEDICINE
    Volume23
    Issue number3
    DOIs
    Publication statusPublished - Mar 2019

    Keywords

    • apoptosis
    • DNA damage
    • p53
    • small intestine
    • total body irradiation

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