The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism

Yu Song; Gang Li; Jacqueline Nowak; Xiaoqing Zhang; Dongbei Xu; Xiujuan Yang; Guoqiang Huang; Wanqi Liang; Litao Yang; Canhua Wang; Vincent Bulone; Zoran Nikoloski; Jianping Hu; Staffan Persson; Dabing Zhang

doi:10.1104/pp.19.00497

The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism

Yu Song, Gang Li, Jacqueline Nowak, Xiaoqing Zhang, Dongbei Xu, Xiujuan Yang, Guoqiang Huang, Wanqi Liang, Litao Yang, Canhua Wang, Vincent Bulone, Zoran Nikoloski, Jianping Hu, Staffan Persson, Dabing Zhang

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Abstract

Light and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directly repressed by the phytochrome-interacting factor-like protein OsPIL16. Consequently, overexpression of OsPIL16 led to gravisensing and actin patterning defects that phenocopied the rmd mutant. Our findings outline a mechanism that links light signaling and gravity perception for straight shoot growth in rice.

Original language	English
Pages (from-to)	630-644
Number of pages	15
Journal	Plant Physiology
Volume	181
Issue number	2
DOIs	https://doi.org/10.1104/pp.19.00497
Publication status	Published - Oct 2019
Externally published	Yes

Bibliographical note

Funding Information:
1This work was supported by the National Key Research and Development Program of China (grant no. 2016YFD0100804), the National Natural Science Foundation of China (grant no. 31430009), the Innovative Research Team, Ministry of Education, and 111 Project (grant no. B14016), the Science and Technology Commission of Shanghai Municipality (grant no. 13JC1408200), the China Scholarship Council (CSC grant no. 201506230050), the Australian Research Council Future Fellowship (grant no. FT160100218 to S.P.), and the University of Melbourne International Research and Research Training Fund-Research Network and Consortia (grant to S.P.). 2Author for contact: [email protected]. 3Senior author.

Publisher Copyright:
Copyright © 2019 American Society of Plant Biologists. All rights reserved

Keywords

Rice
Actin-Binding
Protein
Gravitropism1

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10.1104/pp.19.00497Licence: CC BY

Published versionFinal published version, 2.14 MBLicence: CC BY

Cite this

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title = "The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism",

abstract = "Light and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directly repressed by the phytochrome-interacting factor-like protein OsPIL16. Consequently, overexpression of OsPIL16 led to gravisensing and actin patterning defects that phenocopied the rmd mutant. Our findings outline a mechanism that links light signaling and gravity perception for straight shoot growth in rice.",

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author = "Yu Song and Gang Li and Jacqueline Nowak and Xiaoqing Zhang and Dongbei Xu and Xiujuan Yang and Guoqiang Huang and Wanqi Liang and Litao Yang and Canhua Wang and Vincent Bulone and Zoran Nikoloski and Jianping Hu and Staffan Persson and Dabing Zhang",

note = "Funding Information: 1This work was supported by the National Key Research and Development Program of China (grant no. 2016YFD0100804), the National Natural Science Foundation of China (grant no. 31430009), the Innovative Research Team, Ministry of Education, and 111 Project (grant no. B14016), the Science and Technology Commission of Shanghai Municipality (grant no. 13JC1408200), the China Scholarship Council (CSC grant no. 201506230050), the Australian Research Council Future Fellowship (grant no. FT160100218 to S.P.), and the University of Melbourne International Research and Research Training Fund-Research Network and Consortia (grant to S.P.). 2Author for contact: [email protected]. 3Senior author. Publisher Copyright: Copyright {\textcopyright} 2019 American Society of Plant Biologists. All rights reserved",

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doi = "10.1104/pp.19.00497",

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AU - Yang, Xiujuan

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AU - Wang, Canhua

AU - Bulone, Vincent

AU - Nikoloski, Zoran

AU - Hu, Jianping

AU - Persson, Staffan

AU - Zhang, Dabing

N1 - Funding Information: 1This work was supported by the National Key Research and Development Program of China (grant no. 2016YFD0100804), the National Natural Science Foundation of China (grant no. 31430009), the Innovative Research Team, Ministry of Education, and 111 Project (grant no. B14016), the Science and Technology Commission of Shanghai Municipality (grant no. 13JC1408200), the China Scholarship Council (CSC grant no. 201506230050), the Australian Research Council Future Fellowship (grant no. FT160100218 to S.P.), and the University of Melbourne International Research and Research Training Fund-Research Network and Consortia (grant to S.P.). 2Author for contact: [email protected]. 3Senior author. Publisher Copyright: Copyright © 2019 American Society of Plant Biologists. All rights reserved

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N2 - Light and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directly repressed by the phytochrome-interacting factor-like protein OsPIL16. Consequently, overexpression of OsPIL16 led to gravisensing and actin patterning defects that phenocopied the rmd mutant. Our findings outline a mechanism that links light signaling and gravity perception for straight shoot growth in rice.

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The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism

Abstract

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Keywords

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