TY - JOUR
T1 - The Role of Controlled Surface Topography and Chemistry on Mouse Embryonic Stem Cell Attachment, Growth and Self-Renewal
AU - Macgregor, Melanie
AU - Williams, Rachel
AU - Downes, Joni
AU - Bachhuka, Akash
AU - Vasilev, Krasimir
PY - 2017/9/14
Y1 - 2017/9/14
N2 - The success of stem cell therapies relies heavily on our ability to control their fate in vitro during expansion to ensure an appropriate supply. The biophysical properties of the cell culture environment have been recognised as a potent stimuli influencing cellular behaviour. In this work we used advanced plasma-based techniques to generate model culture substrates with controlled nanotopographical features of 16 nm, 38 nm and 68 nm in magnitude, and three differently tailored surface chemical functionalities. The effect of these two surface properties on the adhesion, spreading, and self-renewal of mouse embryonic stem cells (mESCs) were assessed. The results demonstrated that physical and chemical cues influenced the behaviour of these stem cells in in vitro culture in different ways. The size of the nanotopographical features impacted on the cell adhesion, spreading and proliferation, while the chemistry influenced the cell self-renewal and differentiation.
AB - The success of stem cell therapies relies heavily on our ability to control their fate in vitro during expansion to ensure an appropriate supply. The biophysical properties of the cell culture environment have been recognised as a potent stimuli influencing cellular behaviour. In this work we used advanced plasma-based techniques to generate model culture substrates with controlled nanotopographical features of 16 nm, 38 nm and 68 nm in magnitude, and three differently tailored surface chemical functionalities. The effect of these two surface properties on the adhesion, spreading, and self-renewal of mouse embryonic stem cells (mESCs) were assessed. The results demonstrated that physical and chemical cues influenced the behaviour of these stem cells in in vitro culture in different ways. The size of the nanotopographical features impacted on the cell adhesion, spreading and proliferation, while the chemistry influenced the cell self-renewal and differentiation.
KW - Extra Cellular Matrix chemical cues
KW - Extra Cellular Matrix physical cues
KW - Fibronectin
KW - Mouse embryonic stem cells
KW - Nanotopography
KW - Plasma polymer
KW - Polyacrylic acid
KW - Polyallylamine
KW - Polyoctadiene
UR - http://www.scopus.com/inward/record.url?scp=85029514779&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/DP15104212
UR - http://purl.org/au-research/grants/NHMRC/1122825
UR - http://purl.org/au-research/grants/NHMRC/1032738
U2 - 10.3390/ma10091081
DO - 10.3390/ma10091081
M3 - Article
AN - SCOPUS:85029514779
VL - 10
JO - Materials
JF - Materials
SN - 1996-1944
IS - 9
M1 - 1081
ER -