TY - JOUR
T1 - The Role of Surface Nanotopography and Chemistry on Primary Neutrophil and Macrophage Cellular Responses
AU - Christo, Susan N.
AU - Bachhuka, Akash
AU - Diener, Kerrilyn R.
AU - Mierczynska, Agnieszka
AU - Hayball, John D.
AU - Vasilev, Krasimir
PY - 2016/4/20
Y1 - 2016/4/20
N2 - Synthetic materials employed for enhancing, replacing, or restoring biological functionality may be compromised by the host immune responses that they evoke. Surface modification has attracted substantial attention as a tool to modulate the host response to synthetic materials; however, how surface nanotopography combined with chemistry affects immune effector cell responses is still poorly understood. To address this open question, a unique set of model surfaces with controlled surface nanotopography in the range of 16, 38, and 68 nm has been generated. Tailored outermost surface chemistry that was amine, carboxyl, or methyl group rich has been provided. The combinations of these properties yield 12 surface types that are subject to functional assays assessing key immune effector cells, namely, primary neutrophil and macrophage responses in vitro. The data demonstrate that surface nanotopography leads to enhanced matrix metalloproteinase-9 production from primary neutrophils, and a decrease in pro-inflammatory cytokine secretion from primary macrophages. Together, these results are the first to directly compare the immunomodulatory effects of the cooperative interplay between surface nanotopography and chemistry.
AB - Synthetic materials employed for enhancing, replacing, or restoring biological functionality may be compromised by the host immune responses that they evoke. Surface modification has attracted substantial attention as a tool to modulate the host response to synthetic materials; however, how surface nanotopography combined with chemistry affects immune effector cell responses is still poorly understood. To address this open question, a unique set of model surfaces with controlled surface nanotopography in the range of 16, 38, and 68 nm has been generated. Tailored outermost surface chemistry that was amine, carboxyl, or methyl group rich has been provided. The combinations of these properties yield 12 surface types that are subject to functional assays assessing key immune effector cells, namely, primary neutrophil and macrophage responses in vitro. The data demonstrate that surface nanotopography leads to enhanced matrix metalloproteinase-9 production from primary neutrophils, and a decrease in pro-inflammatory cytokine secretion from primary macrophages. Together, these results are the first to directly compare the immunomodulatory effects of the cooperative interplay between surface nanotopography and chemistry.
KW - Immunomodulation
KW - Macrophages
KW - Neutrophils
KW - Surface chemistries
KW - Surface nanotopographies
UR - http://www.scopus.com/inward/record.url?scp=84959048608&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/NHMRC/631931
UR - http://purl.org/au-research/grants/ARC/DP15104212
U2 - 10.1002/adhm.201500845
DO - 10.1002/adhm.201500845
M3 - Article
C2 - 26845244
AN - SCOPUS:84959048608
SN - 2192-2640
VL - 5
SP - 956
EP - 965
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 8
ER -