The role of the 2- and 3-hydroxyl groups of 1D-myo-inositol 1,4,5-trisphosphate in the mobilisation of calcium from permeabilised human 1321N1 astrocytoma cells

Robert A. Wilcox, Stefan R. Nahorski, Deborah A. Sawyer, Changsheng Liu, Barry V.L. Potter

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The functional significance of the 2- and 3-hydroxyl groups of 1d-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] was probed by using Ins(1,4,5)P3 analogues variously modified at positions 2 and 3 or elsewhere. The intrinsic activities of these compounds were compared to that of Ins(1,4,5)P3, using the calcium-mobilising receptor of the 1321N1 human astrocytoma cell line. The ligand-binding affinities were also determined using membrane preparations from rat cerebellum and bovine adrenal cortex. The results show that HO-2 and HO-3 of Ins(1,4,5)P3 have a relatively insignificant role in receptor binding and calcium release. However, the possibility of a regulatory role for the 3-position of Ins(1,4,5)P3 in these processes is proposed.

Original languageEnglish
Pages (from-to)237-246
Number of pages10
JournalCarbohydrate Research
Volume234
DOIs
Publication statusPublished - 9 Oct 1992
Externally publishedYes

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