The simultaneous determination of theophylline, theobromine and caffeine in plasma by high performance liquid chromatography

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A high performance liquid chromatographic procedure for the simultaneous micro-scale determination of theophylline, theobromine and caffeine in plasma is described. After a single dichloromethane extraction of 0.05 - 0.2 ml of acidified plasma, the evaporated residue is chromatographed on a reverse-phase gC-18) column. With a mobile phase of acetate buffer (pH 4) - acetonitrile (88:12) at a flow-rate of 2.0 ml/min., the three methylxanthines are separated within six minutes. Detection at 276-280 nm enables quantitation of 0.1 mg/l of drug in a 0.1 ml sample. The method is reproducible, correlates well with EMIT for plasma theophylline, and is applicable to the routine monitoring of both paediatric and adult patients as well as to metabolic studies.

Original languageEnglish
Pages (from-to)132-134
Number of pages3
JournalClinical Biochemistry
Volume13
Issue number3
DOIs
Publication statusPublished - Jun 1980
Externally publishedYes

Fingerprint Dive into the research topics of 'The simultaneous determination of theophylline, theobromine and caffeine in plasma by high performance liquid chromatography'. Together they form a unique fingerprint.

  • Cite this