The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections

Malcolm D. Smith, Michael Ahern, Mark Coleman

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

This chapter explores the combination of a nonradioactive in situ hybridization technique to detect mRNA with an imunnohistochemical labeling technique for use in formalin-fixed, paraffin-embedded, or frozen tissue sections. This technique allows the combination of detecting mRNA by in situ hybridization with immunohistochemical detection of a protein product or a cell surface marker without using any radioactive procedures. This technique is ideal for use on tissue sections when the aim is to identify which cells are producing a secreted protein product, such as a cytokine.

Original languageEnglish
Title of host publicationIn Situ Hybridization Protocols
EditorsI.A. Darby, T.D. Hewitson
PublisherHUMANA PRESS
Pages235-245
Number of pages11
ISBN (Electronic)9781597450072
ISBN (Print)9781588294029
DOIs
Publication statusPublished - 2006
Externally publishedYes

Publication series

NameMethods in Molecular Biology™
Volume326
ISSN (Print)1064-3745

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    Smith, M. D., Ahern, M., & Coleman, M. (2006). The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections. In I. A. Darby, & T. D. Hewitson (Eds.), In Situ Hybridization Protocols (pp. 235-245). (Methods in Molecular Biology™; Vol. 326). HUMANA PRESS. https://doi.org/10.1385/1-59745-007-3:235