The use of culture-independent tools to characterize bacteria in endo-tracheal aspirates from pre-term infants at risk of bronchopulmonary dysplasia

Franziska Stressmann, Gary Connett, Kevin Goss, Tanoj Kollamparambil, Nilesh Patel, Matthew Payne, Victoria Puddy, Julian Legg, Kenneth Bruce, Geraint Rogers

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Although premature infants are increasingly surviving the neonatal period, up to one-third develop bronchopulmonary dysplasia (BPD). Despite evidence that bacterial colonization of the neonatal respiratory tract by certain bacteria may be a risk factor in BPD development, little is known about the role these bacteria play. The aim of this study was to investigate the use of culture-independent molecular profiling methodologies to identify potential etiological agents in neonatal airway secretions. This study used terminal restriction fragment length polymorphism (T-RFLP) and clone sequence analyses to characterize bacterial species in endo-tracheal (ET) aspirates from eight intubated pre-term infants. A wide range of different bacteria was identified in the samples. Forty- seven T-RF band lengths were resolved in the sample set, with a range of 0-15 separate species in each patient. Clone sequence analyses confirmed the identity of individual species detected by T-RFLP. We speculate that the identification of known opportunistic pathogens including S. aureus, Enterobacter sp., Moraxella catarrhalis, Pseudomonas aeruginosa.

    Original languageEnglish
    Pages (from-to)333-337
    Number of pages5
    JournalJournal of Perinatal Medicine
    Volume38
    Issue number3
    DOIs
    Publication statusPublished - 2010

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