Abstract
A new technique for localising the centre of sites of microinjection of drugs in the brain using the silver intensification of colloidal gold is described. The technique is compatible with immunocytochemistry and in this study was used in Wistar Kyoto rats to investigate the relationship between the location of phenylethanolamine-N- methyltransferase-like immunoreactive (PNMT-LI) cell bodies in the medulla and the precise sites where microinjection of l-glutamate elicited pressor responses. Colloidal gold provided a simple technique for localising the centre of sites of microinjection with a high degree of spatial resolution and allowed pressor sites to be precisely localised in the region of ventrolateral medulla with the highest density of PNMT-LI cells.
Original language | English |
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Pages (from-to) | 125-130 |
Number of pages | 6 |
Journal | Neuroscience letters |
Volume | 77 |
Issue number | 2 |
DOIs | |
Publication status | Published - 15 Jun 1987 |
Keywords
- Blood pressure
- Colloidal gold
- Glutamate
- Medulla oblongata
- Microinjection of drugs
- Phenylethanolamine-N-methyltransferase
- Rat