Thermal treatment of nasopharyngeal samples before cobas SARS-CoV-2 testing

Todd M. Pryce; Peter A. Boan; Ian D. Kay; James P. Flexman

doi:10.1016/j.cmi.2020.07.042

Thermal treatment of nasopharyngeal samples before cobas SARS-CoV-2 testing

Todd M. Pryce
, Peter A. Boan
, Ian D. Kay
, James P. Flexman

Research output: Contribution to journal › Letter › peer-review

7 Citations (Scopus)

Abstract

Since the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, we have seen shortages of diagnostic reagents, consumables and personal protective equipment [1,2]. Initially we inactivated samples with guanidine hydrochloride (GuHCl) before SARS-CoV-2 testing [2,3]. Following implementation of cobas SARS-CoV-2 testing (Roche, Basel, Switzerland), we encountered shortages of GuHCl and personal protective equipment. To overcome these issues, we investigated a number of rapid heat treatment steps before cobas testing. Temperatures and durations investigated were based on reports demonstrating the effects of temperature on the viability of SARS-CoV-2 (70°C for 5 minutes) [4] and other coronaviruses.

Original language	English
Pages (from-to)	149-150
Number of pages	2
Journal	Clinical Microbiology and Infection
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.cmi.2020.07.042
Publication status	Published - Jan 2021
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

SARS-Cov-2
Nasopharyngeal
cobas
diagnostic shortages

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10.1016/j.cmi.2020.07.042Licence: In Copyright

Cite this

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title = "Thermal treatment of nasopharyngeal samples before cobas SARS-CoV-2 testing",

abstract = "Since the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, we have seen shortages of diagnostic reagents, consumables and personal protective equipment [1,2]. Initially we inactivated samples with guanidine hydrochloride (GuHCl) before SARS-CoV-2 testing [2,3]. Following implementation of cobas SARS-CoV-2 testing (Roche, Basel, Switzerland), we encountered shortages of GuHCl and personal protective equipment. To overcome these issues, we investigated a number of rapid heat treatment steps before cobas testing. Temperatures and durations investigated were based on reports demonstrating the effects of temperature on the viability of SARS-CoV-2 (70°C for 5 minutes) [4] and other coronaviruses.",

keywords = "SARS-Cov-2, Nasopharyngeal, cobas, diagnostic shortages",

author = "Pryce, \{Todd M.\} and Boan, \{Peter A.\} and Kay, \{Ian D.\} and Flexman, \{James P.\}",

year = "2021",

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doi = "10.1016/j.cmi.2020.07.042",

language = "English",

volume = "27",

pages = "149--150",

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}

TY - JOUR

T1 - Thermal treatment of nasopharyngeal samples before cobas SARS-CoV-2 testing

AU - Pryce, Todd M.

AU - Boan, Peter A.

AU - Kay, Ian D.

AU - Flexman, James P.

PY - 2021/1

Y1 - 2021/1

N2 - Since the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, we have seen shortages of diagnostic reagents, consumables and personal protective equipment [1,2]. Initially we inactivated samples with guanidine hydrochloride (GuHCl) before SARS-CoV-2 testing [2,3]. Following implementation of cobas SARS-CoV-2 testing (Roche, Basel, Switzerland), we encountered shortages of GuHCl and personal protective equipment. To overcome these issues, we investigated a number of rapid heat treatment steps before cobas testing. Temperatures and durations investigated were based on reports demonstrating the effects of temperature on the viability of SARS-CoV-2 (70°C for 5 minutes) [4] and other coronaviruses.

AB - Since the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, we have seen shortages of diagnostic reagents, consumables and personal protective equipment [1,2]. Initially we inactivated samples with guanidine hydrochloride (GuHCl) before SARS-CoV-2 testing [2,3]. Following implementation of cobas SARS-CoV-2 testing (Roche, Basel, Switzerland), we encountered shortages of GuHCl and personal protective equipment. To overcome these issues, we investigated a number of rapid heat treatment steps before cobas testing. Temperatures and durations investigated were based on reports demonstrating the effects of temperature on the viability of SARS-CoV-2 (70°C for 5 minutes) [4] and other coronaviruses.

KW - SARS-Cov-2

KW - Nasopharyngeal

KW - cobas

KW - diagnostic shortages

UR - https://www.scopus.com/pages/publications/85090316304

U2 - 10.1016/j.cmi.2020.07.042

DO - 10.1016/j.cmi.2020.07.042

M3 - Letter

C2 - 32771645

AN - SCOPUS:85090316304

SN - 1198-743X

VL - 27

SP - 149

EP - 150

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 1

ER -

Thermal treatment of nasopharyngeal samples before cobas SARS-CoV-2 testing

Abstract

UN SDGs

Keywords

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