Thermodynamic and structural studies of mixed monolayers: Mutual mixing of DPPC and DPPG with DoTAP at the air-water interface

Amiya Kumar Panda, Krasimir Vasilev, Sandra Orgeig, Clive A. Prestidge

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17 Citations (Scopus)


Phospholipid monomolecular films at the air-water interface are useful model membranes to understand miscibility among various components. Surface pressure (π)-area (A) isotherms of pure and mixed monolayers of dioleoyltrimethylammonium propane (DoTAP)-dipalmitoylphosphatidylcholine (DPPC) and DoTAP-dipalmitoyphosphatidylglycerol (DPPG) were constructed using a surface balance. DPPC and DPPG produced isotherms as expected and reported earlier. DoTAP, an unsaturated lipid, demonstrated a continuous π-A isotherm. Associative interactions were identified in DPPC-DoTAP mixtures compared to the pure components, while DPPG-DoTAP mixtures showed repulsive interaction up to an equimolar ratio. Compression moduli of the monolayers revealed that DPPC-DoTAP mixtures had increasing stability with increasing surface pressure, but addition of DoTAP to DPPG showed instability at low and intermediate concentrations. In both cases increased stability was returned at higher XDoTAP values and surface pressures. Lipid monolayer film thickness values, determined on a gold coated glass substrate by surface plasmon resonance spectroscopy (SPR), indicated a systematic change in height profile for DPPC-DoTAP mixtures with increasing XDoTAP. However, DPPG-DoTAP mixed monolayer systems demonstrated a biphasic response. The SPR data were in excellent agreement with our interpretation of the structure of solid supported lipid monolayers.

Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalMaterials Science and Engineering C
Issue number4
Publication statusPublished - 10 May 2010
Externally publishedYes


  • Compressibility modulus
  • Free energy
  • Lift-off area
  • Monolayer
  • Phospholipid
  • Surface plasmon resonance


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