Three-Dimensional Organization of Self-Encapsulating Gluconobacter oxydans Bacterial Cells

Vi Khanh Truong, Chris M. Bhadra, Andrew J. Christofferson, Irene Yarovsky, Mohammad Al Kobaisi, Christopher J. Garvey, Olga N. Ponamoreva, Sergey V. Alferov, Valery A. Alferov, Palalle G. Tharushi Perera, Duy H.K. Nguyen, Ričardas Buividas, Saulius Juodkazis, Russell J. Crawford, Elena P. Ivanova

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Self-organized bacteria have been the subject of interest for a number of applications, including the construction of microbial fuel cells. In this paper, we describe the formation of a self-organized, three-dimensional network that is constructed using Gluconobacter oxydans B-1280 cells in a hydrogel consisting of poly(vinyl alcohol) (PVA) with N-vinyl pyrrolidone (VP) as a cross-linker, in which the bacterial cells are organized in a particular side-by-side alignment. We demonstrated that nonmotile G. oxydans cells are able to reorganize themselves, transforming and utilizing PVA-VP polymeric networks through the molecular interactions of bacterial extracellular polysaccharide (EPS) components such as acetan, cellulose, dextran, and levan. Molecular dynamics simulations of the G. oxydans EPS components interacting with the hydrogel polymeric network showed that the solvent-exposed loops of PVA-VP extended and engaged in bacterial self-encapsulation.

Original languageEnglish
Pages (from-to)8099-8107
Number of pages9
JournalACS Omega
Issue number11
Publication statusPublished - 30 Nov 2017
Externally publishedYes


  • Carbohydrates
  • Bacteria
  • Fuel cells
  • Cluster chemistry
  • Hydrogels


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