Toehold-Mediated Nonenzymatic DNA Strand Displacement As a Platform for DNA Genotyping

Dmitriy Khodakov; Anastasia Khodakova; Adrian Linacre; Amanda Ellis

doi:10.1021/ja310991r

Toehold-Mediated Nonenzymatic DNA Strand Displacement As a Platform for DNA Genotyping

Dmitriy Khodakov, Anastasia Khodakova, Adrian Linacre, Amanda Ellis

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Toehold-mediated DNA strand displacement provides unique advantages in the construction and manipulation of multidimensional DNA nanostructures as well as nucleic acid sequence analysis. We demonstrate a step change in the use of toehold-mediated DNA strand displacement reactions, where a double-stranded DNA duplex, containing a single-stranded toehold domain, enzymatically generated and then treated as a molecular target for analysis. The approach was successfully implemented for human DNA genotyping, such as gender identification where the amelogenin gene was used as a model target system, and detecting single nucleotide polymorphisms of human mitochondrial DNA. Kinetics of the strand displacement was monitored by the quenched Förster resonance energy transfer effect.

Original language	English
Pages (from-to)	5612-5619
Number of pages	8
Journal	Journal of The American Chemical Society
Volume	135
Issue number	15
DOIs	https://doi.org/10.1021/ja310991r
Publication status	Published - 17 Apr 2013

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abstract = "Toehold-mediated DNA strand displacement provides unique advantages in the construction and manipulation of multidimensional DNA nanostructures as well as nucleic acid sequence analysis. We demonstrate a step change in the use of toehold-mediated DNA strand displacement reactions, where a double-stranded DNA duplex, containing a single-stranded toehold domain, enzymatically generated and then treated as a molecular target for analysis. The approach was successfully implemented for human DNA genotyping, such as gender identification where the amelogenin gene was used as a model target system, and detecting single nucleotide polymorphisms of human mitochondrial DNA. Kinetics of the strand displacement was monitored by the quenched F{\"o}rster resonance energy transfer effect.",

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AU - Khodakov, Dmitriy

AU - Khodakova, Anastasia

AU - Linacre, Adrian

AU - Ellis, Amanda

PY - 2013/4/17

Y1 - 2013/4/17

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AB - Toehold-mediated DNA strand displacement provides unique advantages in the construction and manipulation of multidimensional DNA nanostructures as well as nucleic acid sequence analysis. We demonstrate a step change in the use of toehold-mediated DNA strand displacement reactions, where a double-stranded DNA duplex, containing a single-stranded toehold domain, enzymatically generated and then treated as a molecular target for analysis. The approach was successfully implemented for human DNA genotyping, such as gender identification where the amelogenin gene was used as a model target system, and detecting single nucleotide polymorphisms of human mitochondrial DNA. Kinetics of the strand displacement was monitored by the quenched Förster resonance energy transfer effect.

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IS - 15

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Toehold-Mediated Nonenzymatic DNA Strand Displacement As a Platform for DNA Genotyping

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