Toehold-Mediated Nonenzymatic DNA Strand Displacement As a Platform for DNA Genotyping

Dmitriy Khodakov, Anastasia Khodakova, Adrian Linacre, Amanda Ellis

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)

    Abstract

    Toehold-mediated DNA strand displacement provides unique advantages in the construction and manipulation of multidimensional DNA nanostructures as well as nucleic acid sequence analysis. We demonstrate a step change in the use of toehold-mediated DNA strand displacement reactions, where a double-stranded DNA duplex, containing a single-stranded toehold domain, enzymatically generated and then treated as a molecular target for analysis. The approach was successfully implemented for human DNA genotyping, such as gender identification where the amelogenin gene was used as a model target system, and detecting single nucleotide polymorphisms of human mitochondrial DNA. Kinetics of the strand displacement was monitored by the quenched Förster resonance energy transfer effect.

    Original languageEnglish
    Pages (from-to)5612-5619
    Number of pages8
    JournalJournal of The American Chemical Society
    Volume135
    Issue number15
    DOIs
    Publication statusPublished - 17 Apr 2013

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