Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Leisha Richardson, Mirjam Kaestli, Mark Mayo, Jolene Bowers, Apichai Tuanyok, James Schupp, David Engelthaler, David Wagner, Paul Keim, Bart Currie

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

    Original languageEnglish
    Pages (from-to)179-181
    Number of pages3
    JournalJournal of Microbiological Methods
    Volume88
    Issue number1
    DOIs
    Publication statusPublished - Jan 2012

    Keywords

    • Burkholderia pseudomallei
    • Clinical specimen
    • Diagnostic
    • DNA extraction
    • Melioidosis
    • Real-time PCR

    Fingerprint Dive into the research topics of 'Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens'. Together they form a unique fingerprint.

    Cite this