Transcriptional analysis reveals physiological response to acute acidification stress of barramundi Lates calcarifer (Bloch) in coastal areas

Zhenhua Ma; Xing Zheng; Zhengyi Fu; Siqi Lin; Gang Yu; Jian G. Qin

doi:10.1007/s10695-020-00824-6

Transcriptional analysis reveals physiological response to acute acidification stress of barramundi Lates calcarifer (Bloch) in coastal areas

Zhenhua Ma, Xing Zheng, Zhengyi Fu, Siqi Lin, Gang Yu, Jian G. Qin

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

To understand the physiological response of estuarine fish to acidification, barramundi (Lates calcarifer) juveniles were exposed to acidified seawater in experimental conditions. The molecular response of barramundi to acidification stress was assessed by RNA-seq analysis. A total of 2188 genes were identified as differential expression genes. The gene ontology classification system and Kyoto Encyclopedia of Genes and Genomes database analysis showed that acidification caused differential expressions of genes and pathways in the gills of barramundi. Acidification had a great influence on the signal transduction pathway in cell process. Furthermore, we detected that numerous unigenes involved in the pathways associated with lipid metabolism, carbohydrate metabolism, amino acid metabolism, glycan biosynthesis and metabolism specific and non-specific immunity were changed. This study indicates that the physiological responses in barramundi especially the immune system and energy allocation correspond to the variation of environmental pH. This study reveals the necessity for assessment of the potential of estuarine fishes to cope with acidification of the environment and the need to develop strategies for fish conservation in coastal areas.

Original language	English
Pages (from-to)	1729-1741
Number of pages	13
Journal	Fish Physiology and Biochemistry
Volume	46
Issue number	5
DOIs	https://doi.org/10.1007/s10695-020-00824-6
Publication status	Published - Oct 2020

Bibliographical note

Funding Information:
This research was financially supported by the South China Sea Fisheries Research Institute, CAFS (2017ZD01, 2018ZD01), Guangxi Innovation-driven Development Projects (Guike AA18242031), Central Public-interest Scientific Institution Basal Research Fund, and Key Research and Development Plan of Hainan Province (ZDYF2017036, ZDYF2018096).

Publisher Copyright:
© 2020, Springer Nature B.V.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

Acute acidification stress
Coastal areas
Lates calcarifer
Physiological response
Transcriptional analysis

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title = "Transcriptional analysis reveals physiological response to acute acidification stress of barramundi Lates calcarifer (Bloch) in coastal areas",

abstract = "To understand the physiological response of estuarine fish to acidification, barramundi (Lates calcarifer) juveniles were exposed to acidified seawater in experimental conditions. The molecular response of barramundi to acidification stress was assessed by RNA-seq analysis. A total of 2188 genes were identified as differential expression genes. The gene ontology classification system and Kyoto Encyclopedia of Genes and Genomes database analysis showed that acidification caused differential expressions of genes and pathways in the gills of barramundi. Acidification had a great influence on the signal transduction pathway in cell process. Furthermore, we detected that numerous unigenes involved in the pathways associated with lipid metabolism, carbohydrate metabolism, amino acid metabolism, glycan biosynthesis and metabolism specific and non-specific immunity were changed. This study indicates that the physiological responses in barramundi especially the immune system and energy allocation correspond to the variation of environmental pH. This study reveals the necessity for assessment of the potential of estuarine fishes to cope with acidification of the environment and the need to develop strategies for fish conservation in coastal areas.",

keywords = "Acute acidification stress, Coastal areas, Lates calcarifer, Physiological response, Transcriptional analysis",

author = "Zhenhua Ma and Xing Zheng and Zhengyi Fu and Siqi Lin and Gang Yu and Qin, {Jian G.}",

note = "Funding Information: This research was financially supported by the South China Sea Fisheries Research Institute, CAFS (2017ZD01, 2018ZD01), Guangxi Innovation-driven Development Projects (Guike AA18242031), Central Public-interest Scientific Institution Basal Research Fund, and Key Research and Development Plan of Hainan Province (ZDYF2017036, ZDYF2018096). Publisher Copyright: {\textcopyright} 2020, Springer Nature B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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AU - Yu, Gang

AU - Qin, Jian G.

N1 - Funding Information: This research was financially supported by the South China Sea Fisheries Research Institute, CAFS (2017ZD01, 2018ZD01), Guangxi Innovation-driven Development Projects (Guike AA18242031), Central Public-interest Scientific Institution Basal Research Fund, and Key Research and Development Plan of Hainan Province (ZDYF2017036, ZDYF2018096). Publisher Copyright: © 2020, Springer Nature B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/10

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N2 - To understand the physiological response of estuarine fish to acidification, barramundi (Lates calcarifer) juveniles were exposed to acidified seawater in experimental conditions. The molecular response of barramundi to acidification stress was assessed by RNA-seq analysis. A total of 2188 genes were identified as differential expression genes. The gene ontology classification system and Kyoto Encyclopedia of Genes and Genomes database analysis showed that acidification caused differential expressions of genes and pathways in the gills of barramundi. Acidification had a great influence on the signal transduction pathway in cell process. Furthermore, we detected that numerous unigenes involved in the pathways associated with lipid metabolism, carbohydrate metabolism, amino acid metabolism, glycan biosynthesis and metabolism specific and non-specific immunity were changed. This study indicates that the physiological responses in barramundi especially the immune system and energy allocation correspond to the variation of environmental pH. This study reveals the necessity for assessment of the potential of estuarine fishes to cope with acidification of the environment and the need to develop strategies for fish conservation in coastal areas.

AB - To understand the physiological response of estuarine fish to acidification, barramundi (Lates calcarifer) juveniles were exposed to acidified seawater in experimental conditions. The molecular response of barramundi to acidification stress was assessed by RNA-seq analysis. A total of 2188 genes were identified as differential expression genes. The gene ontology classification system and Kyoto Encyclopedia of Genes and Genomes database analysis showed that acidification caused differential expressions of genes and pathways in the gills of barramundi. Acidification had a great influence on the signal transduction pathway in cell process. Furthermore, we detected that numerous unigenes involved in the pathways associated with lipid metabolism, carbohydrate metabolism, amino acid metabolism, glycan biosynthesis and metabolism specific and non-specific immunity were changed. This study indicates that the physiological responses in barramundi especially the immune system and energy allocation correspond to the variation of environmental pH. This study reveals the necessity for assessment of the potential of estuarine fishes to cope with acidification of the environment and the need to develop strategies for fish conservation in coastal areas.

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Transcriptional analysis reveals physiological response to acute acidification stress of barramundi Lates calcarifer (Bloch) in coastal areas

Abstract

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