Transcriptional and epigenetic mechanisms of cellular reprogramming to induced pluripotency

Mark van den Hurk, Gunter Kenis, Cedric Bardy, Daniel van den Hove, Fred Gage, Harry W. M. Steinbusch, Bart Rutten

    Research output: Contribution to journalReview articlepeer-review

    8 Citations (Scopus)

    Abstract

    Enforced ectopic expression of a cocktail of pluripotency-associated genes such as Oct4, Sox2, Klf4 and c-Myc can reprogram somatic cells into induced pluripotent stem cells (iPSCs). The remarkable proliferation ability of iPSCs and their aptitude to redifferentiate into any cell lineage makes these cells a promising tool for generating a variety of human tissue in vitro. Yet, pluripotency induction is an inefficient process, as cells undergoing reprogramming need to overcome developmentally imposed epigenetic barriers. Recent work has shed new light on the molecular mechanisms that drive the reprogramming of somatic cells to iPSCs. Here, we present current knowledge on the transcriptional and epigenetic regulation of pluripotency induction and discuss how variability in epigenetic states impacts iPSCs' inherent biological properties.

    Original languageEnglish
    Pages (from-to)1131-1149
    Number of pages19
    JournalEpigenomics
    Volume8
    Issue number8
    DOIs
    Publication statusPublished - Aug 2016

    Keywords

    • chromatin
    • DNA methylation
    • epigenetics
    • histone modifications
    • induced pluripotent stem cell
    • iPSC
    • pluripotency
    • reprogramming

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