Transfer of bisphenol A across the human placenta

Biju Balakrishnan, Kimiora Henare, Eric B. Thorstensen, Anna P. Ponnampalam, Murray D. Mitchell

Research output: Contribution to journalArticlepeer-review

163 Citations (Scopus)

Abstract

Objective: The objective of the investigation was to study placental transfer and conjugation of bisphenol A (BPA) across the human placenta. Study Design: Human placentae obtained from healthy term singleton pregnancies were utilized in a dual recirculating model of ex vivo placental perfusion. Seven placentae were perfused with BPA (10 ng/mL) added to the maternal perfusate for 180 minutes. Antipyrine and fluorescein isothiocyanate dextran were used as positive and negative controls, respectively, to validate integrity of the circuits. Concentrations of BPA and its conjugates were determined by liquid chromatography-mass spectrometry. Results: The transfer percentage for antipyrine and BPA were 25.5 ± 1.13% and 27.0 ± 1.88%, respectively, and the transfer index for BPA was 1.1 ± 0.09 after 180 minutes of perfusion. Only 3.2 ± 1.6% of BPA in the fetal compartment was in the conjugated form. Conclusion: Bisphenol A at low environmentally relevant levels can transfer across the human placenta, mainly in active unconjugated form.

Original languageEnglish
Pages (from-to)393.e1-393.e7
Number of pages7
JournalAMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
Volume202
Issue number4
DOIs
Publication statusPublished - 1 Apr 2010

Keywords

  • bisphenol A
  • bisphenol A glucuronide
  • placental perfusion
  • placental transfer

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