Translational offsetting as a mode of estrogen receptor α-dependent regulation of gene expression

Julie Lorent, Eric P. Kusnadi, Vincent van Hoef, Richard J. Rebello, Matthew Leibovitch, Johannes Ristau, Shan Chen, Mitchell G. Lawrence, Krzysztof J. Szkop, Baila Samreen, Preetika Balanathan, Francesca Rapino, Pierre Close, Patricia Bukczynska, Karin Scharmann, Itsuhiro Takizawa, Gail P. Risbridger, Luke A. Selth, Sebastian A. Leidel, Qishan LinIvan Topisirovic, Ola Larsson, Luc Furic

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Estrogen receptor alpha (ERα) activity is associated with increased cancer cell proliferation. Studies aiming to understand the impact of ERα on cancer-associated phenotypes have largely been limited to its transcriptional activity. Herein, we demonstrate that ERα coordinates its transcriptional output with selective modulation of mRNA translation. Importantly, translational perturbations caused by depletion of ERα largely manifest as “translational offsetting” of the transcriptome, whereby amounts of translated mRNAs and corresponding protein levels are maintained constant despite changes in mRNA abundance. Transcripts whose levels, but not polysome association, are reduced following ERα depletion lack features which limit translation efficiency including structured 5′UTRs and miRNA target sites. In contrast, mRNAs induced upon ERα depletion whose polysome association remains unaltered are enriched in codons requiring U34-modified tRNAs for efficient decoding. Consistently, ERα regulates levels of U34-modifying enzymes and thereby controls levels of U34-modified tRNAs. These findings unravel a hitherto unprecedented mechanism of ERα-dependent orchestration of transcriptional and translational programs that may be a pervasive mechanism of proteome maintenance in hormone-dependent cancers.

Original languageEnglish
Article numbere101323
Number of pages19
JournalEMBO Journal
Issue number23
Publication statusPublished - 2 Dec 2019
Externally publishedYes


  • 5′ UTR
  • estrogen receptor
  • hormone-dependent cancer
  • mRNA translation
  • U34 tRNA modification


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