TY - JOUR
T1 - Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
AU - Vuong, Thu
AU - Franco, Christopher
AU - Zhang, Wei
PY - 2014/6
Y1 - 2014/6
N2 - Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert.
AB - Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert.
KW - Cell suspension culture
KW - Elicitation
KW - In situ adsorption
KW - Resveratrol
KW - Stilbene
KW - Vitis vinifera
UR - http://www.scopus.com/inward/record.url?scp=84904562828&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2014.04.002
DO - 10.1016/j.btre.2014.04.002
M3 - Article
VL - 1-2
SP - 15
EP - 21
JO - Biotechnology Reports
JF - Biotechnology Reports
SN - 2215-017X
ER -