Trisomy 12 assessment by conventional fluorescence in-situ hybridization (FISH), FISH in suspension (FISH-IS) and laser scanning cytometry (LSC) in chronic lymphocytic leukemia.

Cuc Do, Karen Lower, Cindy Macardle, Bryone Kuss

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Chronic lymphocytic leukemia (CLL) has an extremely heterogeneous clinical course, and prognostication is based on common genetic abnormalities which are detected by standard cytogenetic methods. However, current methods are restricted by the low number of cells able to be analyzed, resulting in the potential to miss clinically relevant sub-clonal populations of cells. A novel high throughput methodology called fluorescence in situ hybridization in suspension (FISH-IS) incorporates a flow cytometry-based imaging approach with automated analysis of thousands of cells. Here we have demonstrated that the FISH-IS technique is applicable to aneuploidy detection in CLL samples for a range of chromosomes using appropriate centromere probes. This method is able to accurately differentiate between monosomy, disomy and trisomy with a sensitivity of 1% in CLL. An analysis comparing conventional FISH, FISH-IS and laser scanning cytometry (LSC) is presented.

    Original languageEnglish
    Pages (from-to)142-149
    Number of pages8
    JournalCancer Genetics and Cytogenetics
    Volume216-217
    DOIs
    Publication statusPublished - Oct 2017

    Keywords

    • centromere
    • chronic lymphocytic leukemia
    • FISH in suspension
    • laser scanning cytometry

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