TY - JOUR
T1 - Tunable morphological changes of asymmetric titanium nanosheets with bactericidal properties
AU - Wandiyanto, Jason V.
AU - Tamanna, Tasnuva
AU - Linklater, Denver P.
AU - Truong, Vi Khanh
AU - Al Kobaisi, Mohammad
AU - Baulin, Vladimir A.
AU - Joudkazis, Saulius
AU - Thissen, Helmut
AU - Crawford, Russell J.
AU - Ivanova, Elena P.
PY - 2020/2/15
Y1 - 2020/2/15
N2 - Hypothesis: Titanium and titanium alloys are often the most popular choice of material for the manufacture of medical implants; however, they remain susceptible to the risk of device-related infection caused by the presence of pathogenic bacteria. Hydrothermal etching of titanium surfaces, to produce random nanosheet topologies, has shown remarkable ability to inactivate pathogenic bacteria via a physical mechanism. We expect that systematic tuning of the nanosheet morphology by controlling fabrication parameters, such as etching time, will allow for optimisation of the surface pattern for superior antibacterial efficacy. Experiments: Using time-dependent hydrothermal processing of bulk titanium, we fabricated bactericidal nanosheets with variable nanoedge morphologies according to a function of etching time. A systematic study was performed to compare the bactericidal efficiency of nanostructured titanium surfaces produced at 0.5, 1, 2, 3, 4, 5, 6, 24 and 60 h of hydrothermal etching. Findings: Titanium surfaces hydrothermally treated for a period of 6 h were found to achieve maximal antibacterial efficiency of 99 ± 3% against Gram-negative Pseudomonas aeruginosa and 90 ± 9% against Gram-positive Staphylococcus aureus bacteria, two common human pathogens. These surfaces exhibited nanosheets with sharp edges of approximately 10 nm. The nanotopographies presented in this work exhibit the most efficient mechano-bactericidal activity against both Gram-negative and Gram-positive bacteria of any nanostructured titanium topography reported thus far.
AB - Hypothesis: Titanium and titanium alloys are often the most popular choice of material for the manufacture of medical implants; however, they remain susceptible to the risk of device-related infection caused by the presence of pathogenic bacteria. Hydrothermal etching of titanium surfaces, to produce random nanosheet topologies, has shown remarkable ability to inactivate pathogenic bacteria via a physical mechanism. We expect that systematic tuning of the nanosheet morphology by controlling fabrication parameters, such as etching time, will allow for optimisation of the surface pattern for superior antibacterial efficacy. Experiments: Using time-dependent hydrothermal processing of bulk titanium, we fabricated bactericidal nanosheets with variable nanoedge morphologies according to a function of etching time. A systematic study was performed to compare the bactericidal efficiency of nanostructured titanium surfaces produced at 0.5, 1, 2, 3, 4, 5, 6, 24 and 60 h of hydrothermal etching. Findings: Titanium surfaces hydrothermally treated for a period of 6 h were found to achieve maximal antibacterial efficiency of 99 ± 3% against Gram-negative Pseudomonas aeruginosa and 90 ± 9% against Gram-positive Staphylococcus aureus bacteria, two common human pathogens. These surfaces exhibited nanosheets with sharp edges of approximately 10 nm. The nanotopographies presented in this work exhibit the most efficient mechano-bactericidal activity against both Gram-negative and Gram-positive bacteria of any nanostructured titanium topography reported thus far.
KW - Antibacterial
KW - Mechano-bactericidal
KW - Nanostructures
UR - http://www.scopus.com/inward/record.url?scp=85075915178&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2019.10.067
DO - 10.1016/j.jcis.2019.10.067
M3 - Article
C2 - 31679779
AN - SCOPUS:85075915178
SN - 0021-9797
VL - 560
SP - 572
EP - 580
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -