TY - JOUR
T1 - Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications
AU - Chen, Zetao
AU - Bachhuka, Akash
AU - Han, Shengwei
AU - Wei, Fei
AU - Lu, Shifeier
AU - Visalakshan, Rahul Madathiparambil
AU - Vasilev, Krasimir
AU - Xiao, Yin
PY - 2017/5/23
Y1 - 2017/5/23
N2 - Osteoimmunomodulation has informed the importance of modulating a favorable osteoimmune environment for successful materials-mediated bone regeneration. Nanotopography is regarded as a valuable strategy for developing advanced bone materials, due to its positive effects on enhancing osteogenic differentiation. In addition to this direct effect on osteoblastic lineage cells, nanotopography also plays a vital role in regulating immune responses, which makes it possible to utilize its immunomodulatory properties to create a favorable osteoimmune environment. Therefore, the aim of this study was to advance the applications of nanotopography with respect to its osteoimmunomodulatory properties, aiming to shed further light on this field. We found that tuning the surface chemistry (amine or acrylic acid) and scale of the nanotopography (16, 38, and 68 nm) significantly modulated the osteoimmune environment, including changes in the expression of inflammatory cytokines, osteoclastic activities, and osteogenic, angiogenic, and fibrogenic factors. The generated osteoimmune environment significantly affected the osteogenic differentiation of bone marrow stromal cells, with carboxyl acid-tailored 68 nm surface nanotopography offering the most promising outcome. This study demonstrated that the osteoimmunomodulation could be manipulated via tuning the chemistry and nanotopography, which implied a valuable strategy to apply a "nanoengineered surface" for the development of advanced bone biomaterials with favorable osteoimmunomodulatory properties.
AB - Osteoimmunomodulation has informed the importance of modulating a favorable osteoimmune environment for successful materials-mediated bone regeneration. Nanotopography is regarded as a valuable strategy for developing advanced bone materials, due to its positive effects on enhancing osteogenic differentiation. In addition to this direct effect on osteoblastic lineage cells, nanotopography also plays a vital role in regulating immune responses, which makes it possible to utilize its immunomodulatory properties to create a favorable osteoimmune environment. Therefore, the aim of this study was to advance the applications of nanotopography with respect to its osteoimmunomodulatory properties, aiming to shed further light on this field. We found that tuning the surface chemistry (amine or acrylic acid) and scale of the nanotopography (16, 38, and 68 nm) significantly modulated the osteoimmune environment, including changes in the expression of inflammatory cytokines, osteoclastic activities, and osteogenic, angiogenic, and fibrogenic factors. The generated osteoimmune environment significantly affected the osteogenic differentiation of bone marrow stromal cells, with carboxyl acid-tailored 68 nm surface nanotopography offering the most promising outcome. This study demonstrated that the osteoimmunomodulation could be manipulated via tuning the chemistry and nanotopography, which implied a valuable strategy to apply a "nanoengineered surface" for the development of advanced bone biomaterials with favorable osteoimmunomodulatory properties.
KW - bone regeneration
KW - inflammation
KW - osteoimmunomodulation
KW - surface chemistry
KW - surface nanotopography
UR - http://www.scopus.com/inward/record.url?scp=85019729225&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/NHMRC/1032738
UR - http://purl.org/au-research/grants/NHMRC/1122825
UR - http://purl.org/au-research/grants/ARC/DP120103697
UR - http://purl.org/au-research/grants/ARC/DP150104212
U2 - 10.1021/acsnano.6b07808
DO - 10.1021/acsnano.6b07808
M3 - Article
C2 - 28414902
AN - SCOPUS:85019729225
SN - 1936-0851
VL - 11
SP - 4494
EP - 4506
JO - ACS Nano
JF - ACS Nano
IS - 5
ER -