UDP glucuronosyltransferase in the cirrhotic rat liver

H. S. Debinski, P. I. MacKenzie, C. S. Lee, M. L. Mashford, J. A. Danks, T. R. Tephly, M. Green, P. V. Desmond

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    In patients with cirrhosis, the elimination of drugs metabolized by glucuronidation is relatively preserved, in comparison with the metabolism of drugs by oxidation. This study explores this phenomenon at a molecular level. In cirrhotic rat livers the content of UDP-glucuronosyltransferase (UGT) was examined by immunohistochemistry and immunoblotting using three antibodies: (i) a polyclonal antibody directed against a broad number of UGT isoforms from both family 1 and family 2; (ii) a family 2-specific antibody; and a (iii) family 1-specific antibody. The steady state mRNA level of UGT of a family 2 isoform was also detected by northern blot analysis. The results demonstrate normal or increased UGT protein by immunohistochemistry and immunoblot in cirrhotic livers compared with controls. This was accompanied by increased steady state mRNA encoding the UGT isoform UGT2B1. In contrast, an isoform of cytochrome P450 (CYP2C11) was reduced markedly in both immunohistochemical staining and immunoblot analysis. These results suggest that in cirrhosis there is a comparative increase or at least a maintenance of UGT enzyme content and that this most likely occurs at a pretranslational level.

    Original languageEnglish
    Pages (from-to)373-379
    Number of pages7
    JournalJournal of Gastroenterology and Hepatology (Australia)
    Volume11
    Issue number4
    DOIs
    Publication statusPublished - Apr 1996

    Keywords

    • carbon tetrachloride
    • cirrhosis
    • UDP glucuronosyltransferase

    Fingerprint Dive into the research topics of 'UDP glucuronosyltransferase in the cirrhotic rat liver'. Together they form a unique fingerprint.

  • Cite this

    Debinski, H. S., MacKenzie, P. I., Lee, C. S., Mashford, M. L., Danks, J. A., Tephly, T. R., Green, M., & Desmond, P. V. (1996). UDP glucuronosyltransferase in the cirrhotic rat liver. Journal of Gastroenterology and Hepatology (Australia), 11(4), 373-379. https://doi.org/10.1111/j.1440-1746.1996.tb01386.x