UDPglucosyltransferase and Its Kinetic Fluorimetric Assay

Matti V.T. VÄISÄNEN, Peter I. MACKENZIE, Osmo O.P. HÄNNINEN

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A rapid, Kinetic assay for UDPglucosyltransferase has been developed using 1‐naphthol as substrate. It is based on the continuous fluorimetric monitoring of 1‐naphthyl glucoside formation during the reaction at physiological pH. The conjugate is easily distinguished from aglycone, since their fluorimetric properties differ. Glucoside biosynthesis in vitro by microsomal preparations isolated from the gut and fat body of cockroaches Periplaneta americana and Leucophaea maderae, and from the green gland and hepatopancreas of the crayfish Astacus astacus, has been demonstrated. The effects of buffer, pH, MgCl2, UDP‐glucuronic acid, UDP‐N‐acetylglucosamine, sodium cholate and sonication on the enzyme activity have been assessed. The kinetic parameters of 1‐naphthol and UDP‐glucose have also been determined.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalEuropean Journal of Biochemistry
Volume130
Issue number1
DOIs
Publication statusPublished - Jan 1983
Externally publishedYes

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