TY - JOUR
T1 - UDPglucosyltransferase and Its Kinetic Fluorimetric Assay
AU - VÄISÄNEN, Matti V.T.
AU - MACKENZIE, Peter I.
AU - HÄNNINEN, Osmo O.P.
PY - 1983/1
Y1 - 1983/1
N2 - A rapid, Kinetic assay for UDPglucosyltransferase has been developed using 1‐naphthol as substrate. It is based on the continuous fluorimetric monitoring of 1‐naphthyl glucoside formation during the reaction at physiological pH. The conjugate is easily distinguished from aglycone, since their fluorimetric properties differ. Glucoside biosynthesis in vitro by microsomal preparations isolated from the gut and fat body of cockroaches Periplaneta americana and Leucophaea maderae, and from the green gland and hepatopancreas of the crayfish Astacus astacus, has been demonstrated. The effects of buffer, pH, MgCl2, UDP‐glucuronic acid, UDP‐N‐acetylglucosamine, sodium cholate and sonication on the enzyme activity have been assessed. The kinetic parameters of 1‐naphthol and UDP‐glucose have also been determined.
AB - A rapid, Kinetic assay for UDPglucosyltransferase has been developed using 1‐naphthol as substrate. It is based on the continuous fluorimetric monitoring of 1‐naphthyl glucoside formation during the reaction at physiological pH. The conjugate is easily distinguished from aglycone, since their fluorimetric properties differ. Glucoside biosynthesis in vitro by microsomal preparations isolated from the gut and fat body of cockroaches Periplaneta americana and Leucophaea maderae, and from the green gland and hepatopancreas of the crayfish Astacus astacus, has been demonstrated. The effects of buffer, pH, MgCl2, UDP‐glucuronic acid, UDP‐N‐acetylglucosamine, sodium cholate and sonication on the enzyme activity have been assessed. The kinetic parameters of 1‐naphthol and UDP‐glucose have also been determined.
UR - http://www.scopus.com/inward/record.url?scp=0020625803&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1983.tb07128.x
DO - 10.1111/j.1432-1033.1983.tb07128.x
M3 - Article
C2 - 6218988
AN - SCOPUS:0020625803
SN - 0014-2956
VL - 130
SP - 141
EP - 145
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -