Ultrastructural localization of lysozyme in human colon eosinophils using the protein A-gold technique: Effects of processing on probe distribution

J. W. Stirling

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The distribution of lysozyme (muramidase) within eosinophil leukocytes situated in the lamina propria of human colon was studied by immunoelectron microscopy using a range of standard techniques. Tissue processed in a variety of glutaraldehyde- or paraformaldehyde-based fixatives was partially dehydrated and embedded in the acrylic resin LR White. Tissue thus treated showed lysozyme in pale cytoplasmic granules and the matrix of specific granules, but not in their crystalloids. Trypsinization of sections had little effect on this result, and tissues fixed in glutaraldehyde and embedded in Araldite showed a low level of reactivity with a similar distribution. After etching LR or Araldite sections with sodium metaperiodate, the pale granules and specific granule matrices became negative for lysozyme and the crystalloids became positive. Because crystalloids also were labeled with noral rabbit immunoglobulin after etching, this apparent redistribution of label could be due to nonspecific binding rather than exposure of masked epitope.

Original languageEnglish
Pages (from-to)709-714
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume37
Issue number5
DOIs
Publication statusPublished - 1 May 1989
Externally publishedYes

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