Under-5-Minute Immunoblot Assays by Vortex Fluidic Device Acceleration

Emily C. Sanders, Sanjana R. Sen, Aidan A. Gelston, Alicia M. Santos, Xuan Luo, Keertna Bhuvan, Derek Y. Tang, Colin L. Raston, Gregory A. Weiss

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Abstract

Unlocking the potential of personalized medicine in point-of-care settings requires a new generation of biomarker and proteomic assays. Ideally, assays could inexpensively perform hundreds of quantitative protein measurements in parallel at the bedsides of patients. This goal greatly exceeds current capabilities. Furthermore, biomarker assays are often challenging to translate from benchtop to clinic due to difficulties achieving and assessing the necessary selectivity, sensitivity, and reproducibility. To address these challenges, we developed an efficient (<5 min), robust (comparatively lower CVs), and inexpensive (decreasing reagent use and cost by >70 %) immunoassay method. Specifically, the immunoblot membrane is dotted with the sample and then developed in a vortex fluidic device (VFD) reactor. All assay steps—blocking, binding, and washing—leverage the unique thin-film microfluidics of the VFD. The approach can accelerate direct, indirect, and sandwich immunoblot assays. The applications demonstrated include assays relevant to both the laboratory and the clinic.

Original languageEnglish
Article numbere202202021
Number of pages6
JournalAngewandte Chemie - International Edition
Early online date25 Mar 2022
DOIs
Publication statusE-pub ahead of print - 25 Mar 2022

Keywords

  • Diagnostics
  • Immunoassay
  • Vortex Fluidics

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